摘要
目的探讨三种检测方法在辽宁省鞍山地区疟疾诊断中的应用价值。方法分别采用镜检、疟原虫抗原快速检测(RDT)胶体金法、巢式PCR法,对鞍山地区2016-2018年16份疟疾疑似病例血样进行检测。结果镜检法、胶体金法、PCR法检测阳性率分别为62.50%、75.00%和68.75%,三种检测方法阳性率比较差异无统计学意义(P=0.748)。以镜检法为金标准,PCR法敏感性为100.00%,特异性为83.33%,以胶体金法为金标准,PCR法敏感性为83.33%,特异性为75.00%,胶体金法敏感性最强。结论在鞍山地区疟疾检测中,RDT胶体金法结合PCR法和镜检法诊断疟原虫,能有效提高诊断率。
Objective To investigate applition value of three testing methods on the diagnosis of malaria in Anshan area.Methods There were 16 blood samples of suspected cases of malaria in Anshan area form 2016 to 2018.Malaria pathogens were examined with microscopy, RDT colloidal gold method and PCR. Results The positive rate of microscopy,RDT colloidal gold method and PCR were 62.50%,75.00% and 68.75%.There was no statistical difference between them(P=0.748). Microscopic examination as the gold standard, the sensitivity and specificity of PCR were 100.00% and 83.33%, respectively. Colloidal gold method as the gold standard, the sensitivity and specificity of PCR were 83.33% and 75.00%, respectively. The colloidal gold method was the most sensitive. Conclusion RDT colloidal gold method combined with PCR and microscopy can be used on the diagnosis of malaria to improve the detection rate in Anshan area.
作者
李明
魏卓超
邓晓丽
许航
LI Ming;WEI Zhuochao;DENG Xiaoli;XU Hang(Anshan Center for Disease Control and Prevention,Anshan,Liaoning 114002, China)
出处
《中国热带医学》
CAS
2019年第6期598-599,共2页
China Tropical Medicine
关键词
疟疾
镜检
胶体金法
巢式聚合酶链反应
Malaria
microscopy
colloidal gold method
nested polymerase chain reaction
