摘要
目的优选大孔吸附树脂纯化铁皮石斛叶总黄酮的工艺条件。方法采用静态吸附-解吸方法,以吸附量和解吸率为指标,优选大孔吸附树脂型号;采用单因素试验分别考察上样浓度、上样体积、洗脱剂及其用量,以铁皮石斛叶总黄酮的纯度为指标,优选大孔吸附树脂纯化铁皮石斛叶总黄酮的最佳工艺条件。结果 ADS-17型大孔吸附树脂对铁皮石斛叶总黄酮的纯化效果最优,最优纯化工艺条件为上样液总黄酮浓度0.7 mg·mL^-1,上样体积30 mL,洗脱剂为50%乙醇,洗脱剂用量为7 BV。总黄酮的纯度为(18.07±0.42)%,回收率为(39.91±0.30)%。结论 ADS-17型大孔吸附树脂在所确定的工艺条件下能有效分离纯化铁皮石斛叶中的总黄酮成分,黄酮纯度提升17倍左右。
OBJECTIVE To investigate the purification technology conditions of total flavones from Dendrobium officinale leaves by macroporous resin. METHODS Taking adsorption and desorption rate as index ingredients, the optimum macroporous resin was screened by static adsorption-elution test;single factor tests were adopted to investigate sample concentration, sample volume, eluting solvent and solvent volume using total flavones contents as an index to optimize conditions for purification of total flavones from Dendrobium officinale by microporous resins. RESULTS ADS-17 macroporous resin was the optimal choice in purification of total flavones from Dendrobium officinale leaves, the ideal technology conditions were as followed: total flavones concentration of sample was 0.7 mg·mL^-1, sample volume was 30 mL,eluting solvent was 50% aqueous ethanol, and eluant volume was 7 BV. the purity of total flavones was(18.07±0.42)%, the yield rate was(39.91±0.30)%. CONCLUSION ADS-17 microporous adsorption resin can effectively separate and purify total flavones from Dendrobium officinale leaves under the determined process condition and the content of flavones was raised by nearly 17 times than before.
作者
娄晓晶
李波
陆婷婷
沈倩
庞敏霞
吕圭源
陈素红
LOU Xiaojing;LI Bo;LU Tingting;SHEN Qian;PANG Minxia;LYU Guiyuan;CHEN Suhong(Zhejiang University of Technology, Hangzhou 310014, China;Zhejiang Chinese Medical University, Hangzhou 310053, China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2019年第11期1338-1342,共5页
Chinese Journal of Modern Applied Pharmacy
基金
国家自然科学基金项目(81673638)
国家重点研发计划项目(2017YFC1702200,2017YFC1702203,2017YFC1702202)
关键词
铁皮石斛叶
总黄酮
大孔吸附树脂
纯化工艺
Dendrobium officinale leaves
total flavones
macroporous resin
purification technology
