摘要
诱导蓝莓丛生芽,筛选出适合蓝莓丛生芽诱导、继代增殖和试管苗生根的最佳培养基。设计正交试验,利用蓝莓幼嫩生长新梢为材料,对蓝莓的丛生芽诱导、增殖和试管苗生根技术进行了研究。诱导蓝莓丛生芽萌发的最适宜培养基为1/2WPM+0.5 mg/L IBA+2.0 mg/L ZT+pH 5.0,诱导丛生芽发芽率为85%,并且长势良好,存活率也比较高;蓝莓丛生芽增殖的适宜培养基为1/2MS+1.0 mg/L ZT+1.5 mg/L 6-BA,丛生芽增殖倍数高达150%,分化的苗数比较多,生长比较旺盛,而且比较粗和高;蓝莓试管苗生根的适宜培养基为改良1/2WPM+1.0 mg/L ZT+0.8 mg/L NAA+pH 5.0+0.25%活性炭,试管苗生根率高达86%,根系比较粗状,分支也比较多。
To screen out the optimum media for the clustered shoot inducing,multiplication and rooting of blueberry plantlets by using orthogonal experimental design.The tender shoot of blueberry was adopted as experimental material.Media in different combination and different concentrations based on orthogonal experimental design were applied to optimize the shoot inducing,multiplication and rooting of blueberry plantlets.Results and conclusion:The results shows that the optimal medium for inducing the sprouting of blueberry clustered shoots is 1/2WPM+0.5 mg/L IBA+2.0 mg/L ZT +pH 5.0.Under this condition the germination percentage is 85% followed by better growth and higher survival rate of blueberry.The most suitable medium for the multiplication of blueberry shoots is 1/2MS+1.0 mg/L ZT+ 1.5 mg/L 6-BA,with a proliferation factor of up to 150%,and under this condition,the plantlets are sturdier and higher.The most suitable medium for rooting of in vitro blueberry plantlet is improved 1/2WPM+1.0 mg/L ZT+0.8 mg/L NAA+pH 5.0+0.25% activated carbon which is followed by rooting rate of 86% with more sturdy roots branches.
作者
陶兴魁
高贵珍
张兴桃
王海潮
李秀娟
孔小燕
TAO Xingkui;GAO Guizhen;ZHANG Xingtao;WANG Haichao;LI Xiujuan;KONG Xiaoyan(School of Biological and Food Engineering,Suzhou University,Suzhou 234000,China)
出处
《安徽工程大学学报》
CAS
2019年第2期1-5,50,共6页
Journal of Anhui Polytechnic University
基金
安徽省教育厅自然科学研究重点基金资助项目(KJ2017ZD36)
宿州学院自然科学研究重点基金资助项目(2016YZD04)
宿州学院特色种植业苗种生产工程技术研究中心开放课题基金资助项目(2017YKF02)
校企合作实践教育基地基金资助项目(2017SJJD032)
关键词
蓝莓
组织培养
培养基筛选
正交试验
vaccinium spp
tissue culture
culture medium optimization
orthogonal design
