摘要
为获得实验室条件下水蒸气蒸馏法提取芳樟精油的优化工艺,采用单因素试验考察不同条件对芳樟精油提取得率的影响,结果表明:芳樟叶片的精油提取得率高于枝干,水上蒸馏方式优于水中蒸馏,最佳蒸馏功率为600 W,最佳蒸馏时间为40 min,叶片采后最佳存放时间为48 h以内,此条件下精油的提取得率为1.42%。采用气相色谱-质谱联用(GC-MS)仪对获得的芳樟精油进行成分分析,鉴定出6种化合物,总GC含量为94.56%,其中主要成分芳樟醇为89.58%。采用菌丝生长速率法测试了不同质量浓度芳樟精油对6种植物病原真菌的抑菌活性,结果表明:芳樟精油对水稻纹枯病菌的抑菌活性较强,质量浓度为500、250和125 mg/L时,抑制率分别为100%、90.88%和67.94%。
In order to investigate the optimal extraction process of Cinnamomum camphora essential oil by steam distillation under laboratory conditions,several factors are investigated by single factor experiments.The results show that the extraction yield of essential oil of the C.camphora leaves is higher than that of branches.The effect of over-water distillation method is better than that of in-water distillation.The optimal distillation power is 600 W,the optimal distillation time is 40 min,and the appropriate post-harvest storage time is less than 48 h.The extraction yield of essential oil under these conditions is 1.42%.The chemical composition of C.camphora essential oil is analyzed by gas chromatograph-mass spectrometer.Six compounds are identified,which account for 94.56%of the total peak area,the main component is linalool,it accounts for 89.58%of the total peak area.The antifungal activities of C.camphora essential oil against six plant pathogenic fungi are tested through determining the growth rate of fungal hyphae.The results show that C.camphora essential oil exhibits good antifungal activity against Rhizoctonia solani,when the testing mass concentration of C.camphora essential oil are 500,250 and 125 mg/L,the inhibition rates are 100%,90.88%and 67.94%,respectively.
作者
郑红富
廖圣良
范国荣
王宗德
陈尚钘
司红燕
ZHENG Hongfu;LIAO Shengliang;FAN Guorong;WANG Zongde;CHEN Shangxing;SI Hongyan(College of Forestry,Jiangxi Agricultural University,National Forestry and Grassland Administration/Jiangxi Camphor Engineering Technology Research Center,Collaborative Innovation Center of Jiangxi Typical Trees Cultivation and Utilization,Nanchang 330045,China)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2019年第3期108-114,共7页
Chemistry and Industry of Forest Products
基金
国家重点研发计划资助项目(2016YFD0600801)
江西省林业厅林业科技创新专项经费项目(201807)
关键词
芳樟
精油
水蒸气蒸馏
化学成分
抑菌活性
Cinnamomum camphora
essential oil
steam distillation
chemical composition
antifungal activity
