摘要
相干拉曼散射具有非侵入、无标记、化学特异性的优点,广泛用于生物组织成像、药代动力学等领域。主要介绍了光纤式相干拉曼散射(CRS)成像光源的实现方式及特点,总结了超连续谱展宽、孤子自频移和四波混频技术在提高双色超短脉冲输出功率、调谐范围、光谱分辨率方面的新进展。报道了基于四波混频的光参量振荡技术在产生可调谐双色超短脉冲方面的最新进展,采用全保偏光纤光路和光子晶体光纤,结合色散滤波和偏振操控技术,获得时间自同步、空间自重合、波长可调谐的双色超短脉冲,可实现脂类、蛋白和核酸的非侵入、无标记光谱检测与成像,为实现结构紧凑、使用方便、环境稳定的CRS提供了一个有效的技术途径。
Coherent Raman scattering is extensively used in various fields, such as biomedical tissue imaging and pharmacokinetics, because of its significant advantages, including non-invasive detection, label-free operation, and chemical specificity. Further, we introduce the implementation and characteristics of the fiber laser sources for coherent Raman scattering(CRS) microscopy, and review the most recent advances in improving the output power, tuning range, and spectral resolution based on the dual-color synchronized ultrashort pulses via supercontinuum generation, soliton self-frequency shift, and four-wave mixing. Additionally, the latest advances in four-wave-mixing-based fiber optical parametric oscillators are introduced. Subsequently, the temporally synchronized, spatially overlapped, and wavelength-tunable dual-color ultrashort pulses are obtained based on dispersion filtering and polarization manipulation using the polarization-maintaining fiber and the photonic crystal fiber. Furthermore, the generated laser pulses can be used to achieve non-invasive as well as label-free spectroscopic detection and microscopic imaging for lipids, proteins, and nucleic acid, which could provide an effective methodology to realize compact, user-friendly, and environmentally stable coherent Raman scattering imaging.
作者
郑世凯
杨康文
敖建鹏
叶蓬勃
郝强
黄坤
季敏标
曾和平
Zheng Shikai;Yang Kangwen;Ao Jianpeng;Ye Pengbo;Hao Qiang;Huang Kun;Ji Minbiao;Zeng Heping(School of Optical-Electrical and Com put er Engineering , University of Shanghai far Science and Technology ,Shanghai 200093, China;State Key Laboratory of Surface Physics and Department of Physics , Fudan University , Shanghai 200433, China;State Key Laboratory of Precision Spectroscopy , East China Normal University , Shanghai 200062, China)
出处
《中国激光》
EI
CAS
CSCD
北大核心
2019年第5期89-99,共11页
Chinese Journal of Lasers
基金
国家自然科学基金(11727812,11504235)
上海高校特聘教授(东方学者)岗位计划(18JC1412000)
关键词
非线性光学
相干反斯托克斯拉曼散射
四波混频
超快激光非线性成像
相干拉曼散射
nonlinear optics
coherent anti-Stokes Raman scattering
four-wave mixing
ultrafast laser nonlinear imaging
coherent Raman scattering
