摘要
目的:探究二十五味珍珠片对斑马鱼微血管功能的改善作用。方法:分别采用花生四烯酸、辛伐他汀、普纳替尼诱导斑马鱼血栓模型,微血管缺失模型以及血管内皮损伤模型。二十五味珍珠片药物干预后,统计斑马鱼心输出量和血流速度,计算斑马鱼肠下血管面积和斑马鱼体节间血管直径,观察尾静脉血栓形成情况,评价二十五味珍珠片对血管内皮的保护作用以及对血栓生成的影响。结果:与模型组比较,二十五味珍珠片27. 8 mg·L^(-1)组心输出量显著性增加(P <0. 05),血流速度显著性增加(P <0. 01);与模型组比较,质量浓度为0. 11,0. 33,1 mg·L^(-1)的二十五味珍珠片干预后,斑马鱼肠下血管面积增加(P <0. 01);血管内皮损伤模型中,与模型组比较,33,100 mg·L^(-1)的二十五味珍珠片干预后,斑马鱼节间血管直径有明显增加(P <0. 05,P <0. 01);与模型组比较,质量浓度为100 mg·L^(-1)的二十五味珍珠片干预后,斑马鱼血栓发生率显著降低(P <0. 01)。结论:二十五味珍珠片可以改善微血管功能障碍,其作用机制可能与其减少血管内皮损伤促进其再生,改善微血管血流动力学作用减少血栓生成有关。
Objective: To explore the effect of Ershiwuwei Zhenzhu tablets( EZT) on microvascular function. Method: The zebrafish models of thrombosis,microvascular defect and vascular endothelial injury were induced by using arachidonic acid,simvastatin and ponatinib respectively,and treated with EZT,astragaloside or asprin. To evaluate the protective effect of EZT on vascular endothelium and its effect on thrombus formation,zebrafish heart output and blood flow velocity were counted,and the vascular area of the zebrafish intestine and the intervascular diameter were calculated. The thrombus in the tail vein was observed under microscope. Result:Compared with model group,EZT improved the cardiac output( P<0. 05) and blood flow velocity( P<0. 01) of zebrafish in thrombus model at a concentration of 27. 8 mg·L^-1,and promoted angiogenesis in zebrafish at concentrations of 0. 11,0. 33,1 mg·L^-1. Compared with the model group,the vascular diameter of the zebrafish internode was significantly increased at the concentrations of 33 mg·L^-1( P<0. 05) and 100 mg·L^-1( P<0. 01),and the incidence of zebrafish thrombosis was significantly reduced after EZT intervention at 100 mg·L^-1( P<0. 01). Conclusion: EZT could improve microvascular dysfunction,and its mechanism may be related to the reduction of vascular endothelial damage to promote its angiogenesis and the improvement of microvascular hemodynamics to reduce thrombus formation.
作者
陈维武
白玛卓玛
郭胜亚
徐懿乔
周生军
陈伦举
CHEN Wei-wu;BAI MA Zhuo-ma;GUO Sheng-ya;XU Yi-qiao;ZHOU Sheng-jun;CHEN Lun-ju(Shenyang Pharmaceutical University, Shenyang 110016, China;Tibet Qizheng Tibetan Medicine Co. Ltd, Lhasa 850030, China;Hunter Biotechnology Inc. , Hangzhou 310051 , China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第13期49-54,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2017ZX09031059)
关键词
二十五味珍珠片
微血管功能
内皮损伤
血栓
Ershiwuwei Zhenzhu tablets
microvascular function
endothelial injury
thrombus
