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镧-大肠杆菌毒物兴奋效应机制探索 被引量:3

A Preliminary Study on Mechanism of Hormesis in Lanthanum-Escherichia Coli
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摘要 为了探索培养液中稀土元素镧浓度变化所引发的大肠杆菌生长的毒物兴奋效应(Hormesis)是否会对菌体内其他无机元素吸收量产生影响,并从菌体中无机元素浓度再平衡的角度探讨并验证Hormesis效应的可能发生机制,特以大肠杆菌为实验菌种,在改变培养液中镧浓度的同时用分光光度计监测大肠杆菌的OD值,藉此观察细菌生长动态;用电感耦合等离子体质谱仪(ICP-MS)测定菌体中45种元素含量并借助统计学方法分析上述元素含量与镧浓度以及细菌生长动态之间的相关性。结果显示,培养液中镧元素浓度变化会引发大肠杆菌生长的Hormesis效应,同时导致菌体内无机元素浓度再平衡;菌体内包括锰在内的部分元素含量随着细菌生长速度的加快而升高,由此推测这些元素在一定浓度范围内或许是有益于细菌生长的"益生元素";培养液中添加锰元素后观察到细菌生长进一步加快,从而初步验证了镧-大肠杆菌Hormesis效应的"益生元素上调机制"的假设。 The aim is to explore whether the hormesis of Escherichia coli(experimental bacteria strain) caused by changing concentration of rare earth elements in the culture fluid will affect the absorption of other inorganic elements by bacteria, and to discuss the possible mechanism of subsequent hormesis response from a rebalancing of inorganic elements in thalli. We altered the lanthanum dosage in the culture and measured absorbance(via spectrophotometry) in Escherichia coli to monitor its growth dynamics. Inductively coupled plasma mass spectrometry(ICP-MS) was used to determine the contents of 45 inorganic elements present in the bacteria. The correlation among the lanthanum dosage, the absorption of these elements and the bacterial growth dynamics was analyzed through statistical methods. The results indicated that changing the dose will cause a hormesis from the growth of E. coli and rebalance of inorganic element in bacteria. The contents of some elements in bacteria, including manganese, will increase as the growth rate of bacteria speeds up. Therefore, the elements mentioned may be the "promoting elements" for bacteria in a certain scope. Adding manganese to the culture can further accelerate the growth of E. coli which preliminarily verifies the hypothesis of "promoting elements" upregulated by adding lanthanum.
作者 高熙 刘雅琼 燕晶晶 杨思宇 赵倩倩 王京宇 Gao Xi;Liu Yaqiong;Yan Jingjing;Yang Siyu;Zhao Qianqian;Wang Jingyu(Departmentof Laboratorial Science and Technology, School of Public Health, Peking University, Beijing100191, China;Peking University Medical and Health Analysis Center, Peking University, VaccineResearch Center, School of Public Health , Peking University, Beijing 100191, China)
出处 《中国稀土学报》 CAS CSCD 北大核心 2019年第3期371-380,I0005,共11页 Journal of the Chinese Society of Rare Earths
关键词 大肠杆菌 毒物兴奋效应 lanthanum manganese Escherichia coli Hormesis
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