摘要
目的建立高效液相色谱法(HPLC)同时测定胎乐颗粒中5种成分(甘草苷、金丝桃苷、黄芩苷、黄芩素和五味子醇甲)的含量。方法梯度洗脱,乙腈-0.1%磷酸水溶液为洗脱体系,检测波长270 nm,总流速1.0 mL/min,进样量10 μL,柱温为30℃。结果甘草苷、金丝桃苷、黄芩苷、黄芩素和五味子醇甲分别在1.96~19.58 μg/mL(r = 0.9999)、1.65~16.52 μg/mL(r = 0.9998)、11.71~117.12 μg/mL(r = 0.9999)、1.63~16.26 μg/mL(r = 0.9999)和1.23~12.28 μg/mL(r = 0.9999)范围内呈良好的线性关系,平均加样回收率(RSD)分别为101.75%(0.98%)、102.32%(1.70%)、98.03%(1.35%)、99.52%(1.54%)和100.12%(1.27%)。结论该方法简单易行,可用于胎乐颗粒的质量控制。
Objective To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination on the contents of five constituents (liquorice, hyperoside, baicalin, baicalein and schisandrin) in Taile Granules. Methods The acetonitrile-0.1% phosphoric acid aqueous solution was eluted by gradient elution. The detection wavelength was 270 nm, the total flow rate was 1.0 mL/min, the injection volume was 10 μL, and the column temperature was 30℃. Results Liquorice, hyperoside, baicalin, baicalein and schisandrin showed good linear relationships within the ranges of 1.96-19.58 μg/mL (r = 0.9999), 1.65-16.52 μg/mL (r = 0.9998), 11.71-117.12 μg/mL (r = 0.9999), 1.63-16.26 μg/mL (r = 0.9999) and 1.23-12.28 μg/mL (r = 0.9999), whose average recoveries (RSD) were 101.75%(0.98%), 102.32%(1.70%), 98.03%(1.35%), 99.52%(1.54%) and 100.12%(1.27%), respectively. Conclusion This method is simple and can be used for the quality control of Taile Granules.
作者
潘涛
欧阳波
蔡霈
杨磊
张志国
肖作奇
PAN Tao;OUYANG Bo;CAI Pei;YANG Lei;ZHANG Zhiguo;XIAO Zuoqi(Department of Pharmaceutical Preparations, Hu′nan Provincial Maternal and Child Health Care Hospital, Hu′nan Province, Changsha 410008, China;Zhang Zhiguo Heritage Studio Related to the Old Chinese Medicine Experts, the First Hospital of Hu′nan University of Chinese Medicine, Hu′nan Province, Changsha 410007, China)
出处
《中国医药导报》
CAS
2019年第15期100-103,118,共5页
China Medical Herald
基金
湖南省中医药管理局科研重点项目(201814)
湖南省长沙市科技计划项目(ZD1702021)
全国名老中医药专家传承工作室建设项目(国中医药人教发[2016]42号)
关键词
胎乐颗粒
高效液相色谱
含量测定
黄芩苷
金丝桃苷
Taile Granules
High performance liquid chromatography
Content determination
Baicalin
Hyperoside
