摘要
目的建立工作场所空气中二苯基甲烷二异氰酸酯(MDI)的浸渍滤纸采集-高效液相色谱测定方法。方法空气中二苯基甲烷二异氰酸酯与浸渍滤纸上的1-(2-吡啶基)哌嗪反应生成MDI-脲衍生物而被吸附于滤纸上,经洗脱、过滤后,高效液相色谱仪测定。结果二苯基甲烷二异氰酸酯的检出限为0.0038μg/ml,定量限为0.013μg/ml,在0.013~2.000μg/ml范围内线性良好(r=0.9997),方法精密度为3.10%~8.03%(n=6),回收率为96.3%~101.9%,滤纸吸附容量为40.8μgMDI,样品在2℃~8℃避光环境中可保存14d。结论本方法优化MDI标准曲线的配制方法,建立了测定工作场所空气中MDI的浸渍滤纸采集-高效液相色谱法。
Objective To develop a method for determining diphenylmethane diisocyanate in workplace air by HPLC with impregnated filter membrane. Methods MDI in workplace air reacted with 1-(2-pyridyl) piperazine on impregnated filter membrane to form MDI-urea derivatives, after elution and filtration, it was detected by HPLC-UV. Results Limit of detection was 0.0038μg/ml and limit of quantification was 0.013 μg/ml. Good linearity was obtained in the range of 0.013~2.000 μg/ml (r=0.999 7). The precision was 3.10%~8.03%(n=6), while the recovery was 96.3%~101.9%. Asorption capacity of the membrane was 40.8 μg MDI, and could be stored for 14 days in the light-proof environment of 2~8 ℃. Conclusion The method optimized testing steps for MDI's standard curve, and provided good guidance for determination of MDI in workplace air with impregnated fiter membrane.
作者
王翔
王祖兵
赵忠林
Wang Xiang;Wang Zubing;Zhao Zhonglin(Shanghai Institute of Occupational Safety & Health, Shanghai 200041, China)
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2019年第5期389-392,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
