摘要
目的探讨桃叶珊瑚苷通过转化生长因子-β/p38 有丝分裂原激活蛋白激酶(transforminggrowth factor-beta/P38 mitogen-activated protein kinases, TGF-β/P38MAPK)信号通路促进皮肤损伤修复的作用机制。方法将毛囊干细胞(hair follicle stem cells, HFSCs)按随机数字表法分为空白组、阳性组及10-10、 10-9、 10-8、 10-7、 10-6、 10-5、 10-4、 10-3 mol/L 桃叶珊瑚苷组,相应药物干预 24 h 后,采用 MTT法检测各组细胞增殖率;将 HFSCs 细胞分为空白组,阳性组,桃叶珊瑚苷低、中、高剂量组。阳性组加入 5 mol/L 的碱性成纤维生长因子溶液,桃叶珊瑚苷低、中、高剂量组分别加入 10-7、 10-6、 10-5 mol/L 桃叶珊瑚苷溶液进行干预。采用 Western blot 检测细胞中 TGF-β、磷酸化 P38(p-p38)、 p38、 Bax、 Bcl-2 蛋白表达,采用实时定量 PCR 检测各组细胞中 Bax、 Bcl-2 mRNA 表达。结果与空白组比较, 10-7、 10-6、10-5 mol/L 桃叶珊瑚苷组细胞增殖率[(0.418±0.031)、(0.412±0.014)、(0.468±0.024)比(0.353±0.006)]升高(P<0.01);与空白组比较,桃叶珊瑚苷低、中、高剂量组细胞 TGF-β[(0.377±0.027)、(0.338±0.021)、(0.322±0.017)比(0.557±0.017)]、 p-p38[(0.270±0.020)、(0.228±0.013)、(0.216±0.012)比(0.461±0.012)]、 Bax[(0.450±0.017)、(0.365±0.011)、(0.279±0.006)比(0.551±0.015)]蛋白表达下调,Bcl-2[(0.450±0.017)、(0.365±0.011)、(0.279±0.006)比(0.358±0.011)]蛋白表达升高(P<0.01);Bcl-2mRNA[(1.714±0.028)、(2.514±0.054)、(3.382±0.084)比(1.000±0)]表达升高, Bax mRNA[(0.415±0.020)、(0.353±0.090)、(0.235±0.114)比(1.000±0)]表达下降(P<0.01)。结论桃叶珊瑚苷可通过调节 TGF-β/p38MAPK 信号通路减少下游凋亡因子蛋白及基因的表达,从而促进损伤皮肤修复。
Objective To explore the mechanism of transforming growth factor-beta/p38 mitogen-activated protein kinases (TGF-β/P38MAPK) signaling pathway that transformed corallin into a transforming growth factor-beta/ p38 mitogen activated protein kinases (TGF-β/P38MAPK). Methods The hair follicle stem cells (HFSCs) were divided into blank group, positive group and 10-10, 10-9, 10-8, 10-7, 10-6, 10-5, 10-4, 10-3 mol/L mycoralin solution groups according to the random number table method. After 24 h, the cell proliferation rate was determined by MTT assay. The HFSCs were divided into blank group, positive group and low, medium and high dose groups according to the random number table method. The positive group was added with 5 mol/L of alkaline fibroblast growth factor solution, while the low, medium and high dose groups were added with 10-7,10-6 and 10-5 mol/L of mycoralin solution for intervention. The cell proliferation rate was determined by MTT assay. The protein expressions of TGF-β, phosphorylated P38(p-p38), P38, Bax and bcl-2 in cells were detected by Western blot, and mRNA expressions of Bax and bcl-2 in cells of each group were detected by real-time quantitative PCR. Results Compared with the blank group, the productivity of the cells (0.418 ± 0.031, 0.412 ± 0.014, 0.468 ± 0.024 vs. 0.353 ± 0.006) in the 10-7, 10-6 and 10-5 mol/L aucubin groups significantly increased (P<0.01). Compared with the blank group, the protein expression of TGF-β(0.377 ± 0.027, 0.338 ± 0.021, 0.322 ± 0.017 vs. 0.557 ± 0.017), p-p38 (0.270 ± 0.020, 0.228 ± 0.013, 0.216 ± 0.012 vs. 0.461 ± 0.012), Bax (0.450 ± 0.017, 0.365 ± 0.011, 0.279 ± 0.006 vs. 0.551 ± 0.015) in the low, medium and high dose groups significantly down-regulated, while the Bcl-2 (0.450 ± 0.017, 0.365 ± 0.011, 0.279 ± 0.006 vs. 0.358 ± 0.011) protein expression significantly increased. Compared with the blank group, the expression of Bcl-2 (1.714 ± 0.028, 2.514 ± 0.054, 3.382 ± 0.084 vs. 1.000 ± 0) mRNA increased, Bax (0.415 ± 0.020, 0.353 ± 0.090, 0.235 ± 0.114 vs. 1.000 ± 0) mRNA in the low, medium and high dose groups significantly down-regulated (P<0.01). Conclusions By regulating the TGF-β/p38MAPK signaling pathway, mycoralin can reduce the expression of downstream apoptotic factors, and promote the repair of damaged skin.
作者
杨柳
李建民
王业秋
安丽凤
黄静文
薛慧
张丽宏
Yang Liu;Li Jianmin;Wang Yeqiu;An Lifeng;Huang Jingwen;Xue Hui;Zhang Lihong(Jiamusi College, Heilongjiang University of Chinese Medicine, Jiamusi 154007, China)
出处
《国际中医中药杂志》
2019年第6期603-607,共5页
International Journal of Traditional Chinese Medicine
基金
黑龙江省卫生计生委科研项目(2017-577)
黑龙江中医药大学科研基金(051733)
黑龙江省中医药科研项目(ZHY16-102)
黑龙江中医药大学博士创新基金(2013bs03)
黑龙江省自然科学基金(H2015023)
黑龙江省教育厅科学技术研究项目(12541765).
