摘要
目的研究射干苷(tectoridin)、桃叶珊瑚苷(aucubin)对脂多糖(LPS)诱导后人脂肪间充质干细胞(hAMSCs)免疫调控作用的影响。方法通过CCK-8细胞增殖实验和细胞周期检测实验,筛选合适的药物作用浓度;筛选LPS作用浓度,建立炎性反应模型;用ELISA、RT-qPCR检测两者对LPS诱导后hAMSCs分泌促炎因子、抑炎因子的影响。结果50μg/mL射干苷和100μg/mL桃叶珊瑚苷能明显促进hAMSCs增殖(P<0.05,P<0.01);两者均促进LPS诱导后hAMSCs增殖(P<0.001);并且可抑制hAMSCs分泌IL-6、TNF-α、IFN-γ等促炎因子(P<0.05,P<0.01)。结论射干苷、桃叶珊瑚苷可显著抑制LPS诱导的hAMSCs炎性反应。
Objective To study the effects of tectoridin and aucubin on the immune regulation of human adipose mesenchymal stem cells(hAMSCs)induced by lipopolysaccharide(LPS).Methods CCK-8 cell proliferation test and cell cycle test were used to screen the appropriate concentration of drugs;LPS concentration was screened to establish an inflammatory response model;ELISA and RT-qPCR were used to detect the effects of both on the secretion of pro-inflammatory and anti-inflammatory factors by hAMSCs after LPS induction.Results Fiftyμg/mL tectoridin and 100μg/mL aucubin could significantly promote the proliferation of normal hAMSCs(P<0.05,P<0.01).Both of them promoted the proliferation of hAMSCs induced by LPS(P<0.001),and inhibited the secre-tion of IL-6,TNF-α,IFN-γ(P<0.05,P<0.01).Conclusions Tectoridin and aucubin may significantly inhibit LPS-induced hAMSCs inflammatory response.
作者
张乃月
朱榕嘉
尹秀平
王巧玲
韩钦
赵春华
宋坪
ZHANG Nai-yue;ZHU Rong-jia;YIN Xiu-ping;WANG Qiao-Ling;HAN Qin;ZHAO Chun-hua;SONG Ping(Department of Dermatology,Guang Anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053;Beijing Key Laboratory of New Drug Development and Clinical Trial of Stem Cell Therapy(BZ0381)/Center of Excellencein Tissue Engineering,Institute of Basic Medical Sciences CAMS,School of Basic Medicine PUMC,Beijing 100005;Peking Union Medical College Hospital,CAMS & PUMC,Beijing 100730;Qingdao University ,Qingdao 266071,China)
出处
《基础医学与临床》
CSCD
2019年第7期961-966,共6页
Basic and Clinical Medicine
基金
国家自然科学基金(81573984)
国家重点研发计划(2016YFA0101000,2016YFA0101003)
中国医学科学院医学与健康科技创新工程经费(2017-I2M-3-007)
高等学校学科与创新引智计划(B18007)
关键词
人脂肪间充质干细胞
炎性细胞因子
免疫调控
银屑病
human adipose mesenchymal stem cells
inflammatory cytokines
immune regulation
psoriasis
