摘要
目的:探讨miR-184通过JNK信号通路对卵巢癌增殖与凋亡的影响。方法:收集卵巢癌患者的癌组织与癌旁组织标本,荧光定量PCR检测miR-184表达;采用Lipofectamine 2000将miR-184 inhibitor和miR-184 mimic分别转入卵巢癌细胞株OVCAR3,qPCR检测其转染效率,MTT及细胞凋亡实验检测miR-184表达对细胞增殖及凋亡的影响;Westerm blot检测miR-184表达对JNK信号通路中total JNK及p-JNK蛋白的影响。结果:卵巢癌组织中miR-184表达较癌旁组织明显升高,差异有统计学意义(P<0.05);高表达miR-184后,OVCAR3细胞增殖率显著提高,细胞凋亡率显著下降,磷酸化p-JNK表达明显上调(P均<0.05);下调miR-184表达后,OVCAR3细胞增殖率明显下降,细胞凋亡率显著升高,磷酸化p-JNK表达明显下调(P均<0.05)。结论:miR-184可促进卵巢癌细胞增殖并抑制细胞凋亡,其机制可能与JNK信号通路有关。
Objective:To investigate the effect of microRNA-184 on proliferation and apoptosis of ovarian cancer through JNK signaling pathway.Methods:The expression of microRNA-184 in ovarian cancer tissues and adjacent tissues was detected by fluorescent quantitative PCR,and the expression of microRNA-184 inhibitor and microRNA-184 mimic were transfected into ovarian cancer cell line OVCAR3 by Lipofectamine 2000.The transfection efficiency was detected by qPCR,and the effects of microRNA-184 expression on cell proliferation and apoptosis were detected by MTT and cell apoptosis experiments.The expression of microRNA-184 was detected by Western blot and it's effects of alteration on total JNK and p-JNK proteins in JNK signaling pathway.Results:The expression of microRNA-184 in ovarian cancer tissues was significantly higher than that in adjacent tissues(P<0.05).After high microRNA-184,the proliferation rate and apoptotic rate of OVCAR3 cells were significantly increased,and the expression of phosphorylated p-JNK in OVCAR3 cells was significantly increased(P<0.05),while after down-regulation of microRNA-184,the proliferation rate and apoptotic rate of OVCAR3 cells were significantly decreased.The expression of phosphorylated p-JNK was significantly down-regulated(P<0.05).Conclusions:MiR-184 can promote the proliferation and inhibit the apoptosis of ovarian cancer cells,and its mechanism may be related to JNK signaling pathway.
作者
汤锶锶
翁双燕
万丹
张艳
Tang Sisi;Weng Shuangyan;Wan Dan(Department of Gynecology,Chongqing Traditional Chinese Medicine Hospital,Chongqing400020)
出处
《现代妇产科进展》
CSCD
北大核心
2019年第7期498-500,505,共4页
Progress in Obstetrics and Gynecology
基金
重庆市卫生计生委中医药科技项目(No:ZY201702007)
