摘要
背景:重度急性胰腺炎(SAP)以胰腺弥漫性出血和组织坏死为特征,具有很高的死亡率。PEP-1-SOD1是一种利用基因工程技术合成的融合蛋白,稳定性较高,具有一定的抗炎作用。目的:探讨PEP-1-SOD1对SAP大鼠细胞凋亡的影响。方法:将24只雄性Wistar大鼠分为对照组、SAP组和实验组。以5%牛磺胆酸钠制备SAP大鼠模型,实验组在造模前30 min腹部皮下注射8.0 mg/kg PEP-1-SOD1,SAP组注射同剂量0.9%NaC l溶液。行组织病理学评分,以TUNEL法检测胰腺腺泡细胞凋亡情况,分别以荧光定量PCR和蛋白质印迹法检测caspase-3 mRNA和蛋白表达。结果:造模后24 h,SAP组和实验组大鼠血清脂肪酶、淀粉酶水平、caspase-3 mRNA和蛋白表达均显著高于对照组(P<0.05),而实验组血清脂肪酶、淀粉酶显著低于SAP组(P<0.05),caspase-3 mRNA和蛋白表达显著升高(P<0.05)。造模后6 h、24 h,SAP组和实验组胰腺组织病理学评分、凋亡指数显著高于对照组(P<0.05),而实验组组织病理学评分显著低于SAP组(P<0.05),凋亡指数显著升高(P<0.05)。结论:PEP-1-SOD1可通过调控凋亡相关基因caspase-3的表达,减轻SAP大鼠胰腺组织病理损害,增加胰腺腺泡细胞凋亡,促进胰腺功能恢复正常。
Background:Severe acute pancreatitis(SAP)is characterized by diffuse pancreatic hemorrhage and tissue necrosis with high mortality.PEP-1-SOD1 is a fusion protein synthesized by genetic engineering technology.It has a high stability and certain anti-inflammatory effects.Aims:To investigate the effect of PEP-1-SOD1 on cell apoptosis in SAP rats.Methods:A total of 24 male Wistar rats were divided into control group,SAP group and experimental group.SAP rat model was established by infusion of 5%sodium taurocholate.Thirty minutes before the establishment,rats in experimental group were abdominal subcutaneously injected with 8.0 mg/kg PEP-1-SOD1,and rats in SAP group were injected with same dose of 0.9%NaC l solution.Histopathological score of pancreatic tissue were evaluated;apoptosis of pancreatic acinar cell was determined by TUNEL.The mRNA and protein expressions of caspase-3 were detected by fluorescent quantitative PCR and Western blotting,respectively.Results:After 24 hours of model establishment,serum amylase and lipase,mRNA and protein expressions of caspase-3 in SAP group and experimental group were significantly higher than those in the control group(P<0.05),however,serum amylase and lipase in experimental group were significantly lower than those in SAP group(P<0.05),while mRNA and protein expressions of caspase-3 were significantly increased(P<0.05).After 6,24 hours of model establishment,histopathological score,apoptotic index in SAP group and experimental group were significantly higher than those in the control group(P<0.05),however,histopathological score in experimental group was significantly lower than that in SAP group(P<0.05),while apoptotic index was significantly increased(P<0.05).Conclusions:PEP-1-SOD1 may increase the apoptosis of pancreatic acinar cells through regulating the expression of apoptosis related gene caspase-3 in SAP rats,thereby reducing the pathological damage of pancreatic tissue and promoting the recovery of pancreatic function.
作者
吕飞
田书芳
LV Fei;TIAN Shufang(Department of Gastroenterology,Shiyan Renmin Hospital,Shiyan,Hubei Province,442000;Department of Traditional Chinese Medicine,Shiyan Renmin Hospital,Shiyan,Hubei Province,442000)
出处
《胃肠病学》
2019年第5期285-288,共4页
Chinese Journal of Gastroenterology
基金
湖北省卫生厅科研一般项目(JX6B108)
