摘要
目的建立强筋健骨丸的含量测定方法。方法以君药制川乌、制草乌中苯甲酰乌头原碱、苯甲酰次乌头原碱及苯甲酰新乌头原碱为目标成分,采用HPLC法,色谱柱Ultimate XB-C18(250mm×4.6mm,5μm);流动相:乙腈(A)-0.1mol·L^-1醋酸铵溶液(B);流速:1.0mL·min^-1;检测波长:235nm。结果苯甲酰新乌头原碱、苯甲酰乌头原碱和苯甲酰次乌头原碱质量浓度分别在7.580~121.280(r1=0.9999),1.576~25.216(r2=0.9998)和7.732~123.712(r3=0.9999)mg·L^-1范围内线性关系均良好,回收率分别为100.1%,100.0%和99.8%,RSD值分别为0.8%,1.5%和0.6%。结论该方法结果准确、稳定、可靠,能用于强筋健骨丸中苯甲酰新乌头原碱、苯甲酰乌头原碱及苯甲酰次乌头原碱的含量测定,可有效控制产品质量。
Objective To establish a method for the determination of Qiangjin Jiangu Pills.Methods The target components were benzoylaconine,benzoylhypaconine and benzoylmesaconine in the monarch medicine Radix Aconiti preparata and Radix Aconiti Kusnezoffii preparata.HPLC was used with Ultimate XB-C 18(250 mm×4.6 mm,5μm)as the column,and acetonitrile(A)-0.1 mol·L^-1 ammonium acetate solution(B)as the mobile phase.The flow rate was 1.0 mL·min^-1 and the detection wavelength was 235 nm.Results The linear relationships of benzoylmesaconine in the mass concentration range of 7.580-121.280 mg·L^-1(r 1=0.999 9),benzoylaconine in the range of 1.576-25.216 mg·L^-1(r2=0.999 8)and benzoylhypaconine in the range of 7.732-123.712 mg·L^-1(r3=0.999 9)were good.The recovery rates were 100.1%,100.0%and 99.8%,with RSD of 0.8%,1.5%and 0.6%,respectively.Conclusion The method is accurate,stable and reliable,and can be used to determine the content of benzoylaconine,benzoylhypaconine and benzoylmesaconine in Qiangjin Jiangu Pills and control the product quality effectively.
作者
霍彬科
黄安军
HUO Binke;HUANG Anjun(Baoji Food and Drug Testing Center,Baoji 721013,China)
出处
《西北药学杂志》
CAS
2019年第4期483-486,共4页
Northwest Pharmaceutical Journal
