摘要
[目的]从宁夏中卫硒砂瓜种植区多年连作土壤中分离获得1株高效降解酚酸类物质的菌株,对其进行分类鉴定,并全面评估其对11种酚酸类物质的降解效能,以期为该菌在酚酸类自毒物质降解领域中的应用提供理论依据。[方法]通过形态特征和16SrRNA基因序列分析进行菌株鉴定;采用液相色谱串联二极管阵列检测器研究菌株对多种酚酸物质的降解特性。[结果]经生理生化及分子生物学鉴定,该菌株为不动杆菌属(Acinetobactersp.),命名为K7。该菌株对4-羟基苯甲酸、香豆酸、香草酸、苯甲酸、阿魏酸和咖啡酸具有良好的降解效果,对丁香酸、香草醛、肉桂酸和没食子酸的降解效能较弱,不能降解水杨酸。以咖啡酸为代表,研究不同条件对菌株K7降解效能的影响,发现在中性偏碱(pH7~8)、较高温度(30~40℃)、咖啡酸初始浓度小于100mg·L-1时,菌株K7对咖啡酸具有高效降解能力。[结论]不动杆菌K7对多种酚酸类化合物表现出良好的降解效能。
[Objectives] A strain which could degrade phenolic acids efficiently was isolated from the continuous cropping soil of watermelon planting area in Ningxia Hui Autonomous Region. Then the identification of the train was conducted. The degradation efficiency of the strain to eleven phenolic acids was evaluated integrally. The result could provide useful information for the application of the strain to weaken autotoxicity caused by the phenolic acids.[Methods] The taxonomic status of the strain was identified by the partial 16S rRNA gene sequence. The degradation characters of strain to the phenolic acids was detected by the HPLC-DAD.[Results] The strain was identified as Acinetobacter sp. and named K7. 4-hydroxybenzoic acid,coumalic acid,vanillic acid,benzoic acid,ferulic acid and caffeic acid were degraded by the strain K7 quickly;while the degradation efficiency of strain K7 to syringic acid,vanillic aldehyde ,cinnamic acid and gallic acid were weak,and no degradation was showed for salicylic acid. Then,as a representative compound ,caffeic acid was used to study the effect of different conditions on the degradation efficiency of strain K7. It was found that high degradation efficiency was obtained at pH7-pH8,temperature at 30-40 ℃ and the initial concentration of caffeic acid was less than 100 mg · L^-1 .[Conclusions] The K7 strain showed good degradation efficiency for various phenolic acids.
作者
李敏
屈欢
刘建利
代秀秀
司海丽
张琇
杨国平
LI Min;QU Huan;LIU Jianli;DAI Xiuxiu;SI Haili;ZHANG Xiu;YANG Guoping(College of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China;Ningxia Key Laboratory of Microbial Resources Development and Applications in Special Environment,North Minzu University,Yinchuan 750021,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2019年第4期689-696,共8页
Journal of Nanjing Agricultural University
基金
宁夏自然科学基金项目(NZ17091)
中央高校基本科研业务费专项资金项目(2017SKKY01)
北方民族大学引进人才科研启动项目(4400302501)
关键词
酚酸
不动杆菌
生物降解
效能
phenolic acids
Acinetobacter sp.
biodegradation
efficiency
