摘要
目的评价纸片扩散法与微量肉汤稀释法检测念珠菌对氟康唑和伏立康唑药物敏感性的一致率。方法针对218株侵袭性真菌感染部位分离的念珠菌,分别采用美国临床和实验室标准协会(CLSI)M44-A2纸片扩散法和CLSIM27微量肉汤稀释法进行药物敏感性检测。依据2018年颁布的CLSIM60新的纸片扩散法判定折点,以微量肉汤稀释法为标准评估纸片扩散法测定结果的分类一致率和错误率。结果2种方法检测白念珠菌、热带念珠菌、近平滑念珠菌和光滑念珠菌氟康唑药物敏感性的一致率分别为97.6%、95.1%、97.4%和90.5%。2种方法在检测白念珠菌、热带念珠菌、近平滑念珠菌和克柔念珠菌伏立康唑敏感性的一致率分别为97.6%、90.2%、97.4%和81.2%。结论纸片扩散法检测念珠菌对氟康唑和伏立康唑的药物敏感性与微量肉汤稀释法一致率较高,是念珠菌药物敏感性测定简便且准确性较高的检测方法,推荐在真菌实验室常规检测或耐药监测网使用。
Objective To compare the CLSI disk diffusion and broth microdilution (BMD) methods for testing susceptibilities of 218 Candida spp.to fluconazole and voriconazole.Methods All the isolates were collected from patients with invasive fungal infections,and were performed susceptibility test according to CLSI M44 A2 and CLSI M27.The categorical agreement (CA) between two methods was evaluated according to the breakpoints described in CLSI M60.Results The CA between KB and BMD for C.albicans,C.tropicalis,C.parapsilosis and C.glabrata to fluconazole was 97.6%,95.1%,97.4% and 90.5%,respectively.The CA between KB and BMD for C.albicans,C.tropicalis,C.parapsilosis and C.krusei to voriconazole was 97.6%,90.2%,97.4% and 81.2%,respectively.Conclusion Disk diffusion method is a simple and accurate method for the detection of Candida susceptibility to fluconazole and voriconazole.It is recommended to be used in routine bacterial laboratory detection or drug resistance monitoring network.
作者
张丽
王贺
肖盟
徐英春
ZHANG Li;WANG He;XIAO Meng;XU Yingchun(Department of Clinical Laboratory,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China;Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases,Beijing 100730,China)
出处
《国际检验医学杂志》
CAS
2019年第13期1583-1586,1591,共5页
International Journal of Laboratory Medicine
基金
国家自然科学基金项目(81802049)
中央高校基本科研业务(3332018035)
