摘要
目的探讨补阳还五汤对大鼠全脑缺血再灌注L型Ca^2+通道平均开放时间的影响。方法应用四血管闭塞法制备全脑缺血再灌注大鼠62只,采用随机数字表法将其分为对照组和研究组,每组各31只。对照组应用生理盐水灌服,研究组应用补阳还五汤水煎液灌服。在处理后2、12、24、48、72 h五个时间点进行观察。比较两组大鼠不同时间点L型Ca^2+通道平均开放时间、脑组织含水量、血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果研究组大鼠不同时间点L型Ca^2+通道平均开放时间明显短于对照组(P <0.05);与本组再灌注72 h比较,两组其余各时间点L型Ca2+通道平均开放时间差异有统计学意义(P <0.05)。缺血再灌注处理后研究组大鼠脑组织含水量也明显低于对照组(P <0.05),与本组再灌注72 h比较,两组其余各时间点脑组织含水量差异有统计学意义(P <0.05)。干预后,研究组血清SOD活性高于对照组,而MDA含量低于对照组(P <0.05)。结论补阳还五汤对大鼠全脑缺血再灌注损伤具有一定治疗作用,其作用与缩短不同时间点L型Ca2+通道平均开放时间、降低大鼠脑组织含水量、增高大鼠血清SOD活性及降低MAD含量有关。
Objective To investigate the effect of Buyang Huanwu Decoction on the average opening time of L-type Ca^2+ channel in rats with global cerebral ischemia-reperfusion. Methods Sixty-two rats with global cerebral ischemia-reperfusion were prepared by four-vessel occlusion method. They were divided into control group and research group by random number table method, with 31 rats in each group. The control group was fed with normal saline, while the research group was fed with Buyang Huanwu Decoction. After 2, 12, 24, 48 and 72 h of treatment, the rats were observed. The average opening time of L-type Ca^2+ channel, brain water content, serum superoxide dismutase (SOD) and malondialdehyde (MDA) levels were compared between the two groups at different time points. Results The average opening time of L-type Ca^2+ channels in the study group was significantly shorter than that in the control group (P < 0.05). Compared with 72 h after reperfusion, the average opening time of L-type Ca^2+ channels in the two groups at different time points were statistically significant (P < 0.05). After ischemia-reperfusion treatment, the water content in the study group was also significantly lower than that in the control group (P < 0.05). Compared with 72 h after reperfusion, the brain water content in the other time points of the two groups were significant difference (P < 0.05). After intervention, serum SOD activity of the study group was higher than that of the control group and the MDA content was lower than that of the control group (P < 0.05). Conclusion Buyang Huanwu Decoction has a certain therapeutic effect on global cerebral ischemia-reperfusion injury in rats. Its effect is related to shortening the average opening time of L-type Ca^2+ channel at different time points, decreasing the brain water content of rat, increasing the activity of serum SOD and decreasing the content of MAD in rat.
作者
梁逸仙
周光辉
廖仲波
赵青
周锐钧
张继平
秦曼妮
LIANG Yixian;ZHOU Guanghui;LIAO Zhongbo;ZHAO Qing;ZhOU Ruijun;ZHANG Jiping;QIN Manni(Department of Clinical Laboratory, Dongguan Third People's Hospital, Guangdong Province, Dongguan 523326,China;Department of Clinical Laboratory, Dongguan Third People's Hospital, Guangdong Province, Dongguan 523326,China)
出处
《中国医药导报》
CAS
2019年第16期13-16,共4页
China Medical Herald
基金
广东省中医药局科研项目(20162145)
关键词
补阳还五汤
大鼠全脑缺血
再灌注
L型Ca^2+通道
药效研究
Buyang Huanwu Decoction
Rat global cerebral ischemia
Reperfusion
L-type Ca^2+ channel
Pharmacodynamic study
