摘要
以对硝基酚(PNP)为目标污染物,利用球形红细菌(Rhodobacter sphaeroides)H菌株研究其对PNP的降解特性,通过单因素实验和响应面分析相结合的方法优化降解条件,以提高H菌株对PNP的降解能力。设置不同反应体系证明了H菌株活细胞是降解PNP主体,且在厌氧光照、厌氧黑暗、好氧光照和好氧黑暗四种条件下均能降解PNP。通过单因素实验得出显著影响因素为:PNP初始浓度、pH值和温度,响应面优化后的最优降解条件为:PNP初始浓度为81.01mg L^-1、pH值8.09和温度30.49℃,PNP降解率的预测值为92.3%,与实际值(91.1%)相差1.2%(<2%),说明预测值可靠。在最优条件下,H菌株的生长和PNP浓度随时间变化关系表明,在H菌株生长的适应期96h内,PNP浓度从81.01mg L^-1降低到20.33mg L^-1,降解率为74.9%,指数生长期96~168h,PNP被快速降解,降解率达到91.1%;同时,拟合了该条件下H菌株降解PNP的一级动力学方程。
With p-nitrophenol(PNP)as the target pollutant,the degradation characteristics of PNP by Rhodobacter sphaeroides H strain were studied. The degradation conditions were optimized by single factor test and response surface analysis,and the degradation ability of H strain to PNP was improved. Different reaction systems have been set up to prove that H strain living cells are the main body of degrading PNP,and can degrade PNP under anaerobic light,anaerobic darkness,aerobic light and aerobic darkness. The single factor experiments show that the significant influencing factors are initial concentration of PNP,pH value and temperature. The optimal degradation conditions after response surface optimization are:initial concentration of PNP is 81.01 mg·L^-1,pH value is 8.09 and temperature is 30.49 ℃. The predicted value of PNP degradation rate is 92.3%,which is 1.2%(<2%)different from the actual value(91.1%). Under the optimum conditions,the relationship between the growth of H strain and the concentration of PNP with time shows that the concentration of PNP decreased from 81.01 mg·L^-1 to 20.33 mg·L^-1 within 96 hours of the growth adaptation period of H strain,and the corresponding degradation rate is 74.9%. Then,in the exponential growth period of 96-168 hours,PNP is rapidly degraded,and the degradation rate reaches 91.1%. At the same time,the first-order kinetic equation of PNP degradation of H strain under this condition was fitted.
作者
孙慧敏
白红娟
张晴
SUN Hui-min;BAI Hong-juan;ZHANG Qing(School of Environment and Safety Engineering,North University of China,Taiyuan 030051,China)
出处
《含能材料》
EI
CAS
CSCD
北大核心
2019年第7期542-549,I0004,共9页
Chinese Journal of Energetic Materials
基金
山西省回国留学人员科研资助项目(2016-084)
