摘要
目的分析活化T细胞核因子1(NFATc1)在肝癌组织中表达及与预后的关系,NFATc1对肝癌细胞迁移能力的影响。方法收集天津市第一中心医院肝胆外科2015年1月至6月33例肝细胞癌患者标本,检测肝癌和癌旁组织NFATc1的表达及分析患者预后。肝癌细胞HepG2随机分为:空白对照组(无任何干预),干扰组(感染慢病毒干扰序列,下调NFATc1表达),阴性对照组(感染慢病毒阴性对照序列)。实时荧光定量聚合酶链反应(RT-PCR)检测患者和细胞NFATc1mRNA的相对表达量,Western印迹检测三组细胞NFATc1、N-钙黏蛋白(N-cadherin)、E-钙黏蛋白(E-cadherin)表达,Transwell检测细胞迁移情况。结果肝癌组织NFATc1mRNA的相对表达量高于癌旁组织,差异有统计学意义(P<0.05)。依据33例患者NFATc1表达的中位数分为高表达组(n=17)和低表达组(n=16)。低表达组的生存率优于高表达组,差异有统计学意义(P<0.05)。干扰组细胞NFATc1mRNA相对表达量低于阴性对照组和空白对照组,差异有统计学意义(P<0.05)。干扰组NFATc1和N-cadherin的蛋白相对表达量低于阴性对照组和空白对照组,而E-cadherin相对表达量高于阴性对照组和空白对照组,差异有统计学意义(P<0.05)。干扰组迁移细胞数(24.0±5.6)个,低于空白对照组[(69.0±4.0)个]和阴性对照组[(73.0±4.4)个],差异有统计学意义(P<0.05)。结论NFATc1在肝癌中表达升高,且高表达的患者预后差。NFATc1低表达降低肝癌细胞迁移能力。
Objective To investigate the function of activated T cell nucleus factor 1 (NFATc1) in liver cancer tissues and the correlations between (NFATc1 and prognosis, and the effect of NFATc1 on liver cancer cell migration. Methods The hepatobiliary surgery department of Tianjin First Central Hospital collected 33 patients with hepatocellular carcinoma from January to June 2015, and studied the expression of NFATc1 in liver cancer and adjacent tissues and the prognosis of patients. HCC cell HepG2 were randomly divided into blank control group (without any intervention), interference group (infected with lentivirus interference sequence, down-regulated expression of NFATc1), and negative control group (infected with lentivirus negative control sequence). The relative expression of NFATc1 in the patients and the three groups were detected by real-time fluorescence quantitative polymerase chain reaction (rt-pcr), and the expressions of NFATc1, n-cadherin and e-cadherin in the three groups were detected by Western blot, and the migration of the cells was detected by Transwell. Results The mRNA level of NFATc1 in liver cancer tissues was higher than that in adjacent tissues, and the difference was statistically significant (P<0.05). According to the median expression of NFATc1, the 33 patients were divided into high-expression group (n=17) and low-expression group (n=16). The survival rate of the high-expression group was better than that of the low-expression group, and the difference was statistically significant (P<0.05). The mRNA level of NFATc1 in the interference group was lower than that in the negative control group and the blank control group, and the difference was statistically significant (P<0.05). The mRNA levels of NFATc1 and n-cadherin in the interference group were lower than those in the negative control group and the blank control group, while the mRNA levels of e-cadherin were higher than those in the negative control group and the blank control group, with statistically significant differences (P<0.05). The number of migrative cells in the interference group was (24.0±5.6), which was lower than that in the blank control group [(69.0±4.0)] and the negative control group [(73.0±4.4)], and the difference was statistically significant (P<0.05). Conclusions The expression of NFATc1 is increased in liver cancer, and high level of NFATc1 indicated poor prognosis in liver cancer patients. Low expression of NFATc1 reduces the migration ability of liver cancer cells.
作者
牛连杰
张雅敏
Niu Lianjie;Zhang Yamin(A Central Clinical College of Tianjin Medical University,Tianjin 300070,China;Department of Hepatobiliary Surgery,Tianjin First Central Hospital,Tianjin 300192,China)
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2019年第6期439-442,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
癌
肝细胞
NFATC转录因子类
预后
肿瘤转移
Carcinoma,hepatocellular
NFATC transcription factors
Prognosis
Neoplasm metastasis
