摘要
目的了解天津地区无偿献血人群人类免疫缺陷病毒(HIV)感染情况,并探讨ELISA检测结果及S/CO值、NAT筛查结果与确证结果相关性。方法分析本实验室2016年9月1日至2017年8月31日献血者血液HIV筛查及确证数据。结果研究期间157130例无偿献血者中292例筛查反应性,其中59例酶联免疫试验(ELISA)双试剂反应性,52例确证阳性(88%);其中56例核酸检测(NAT)反应性(含窗口期标本1例),确证阳性53例(94.6%);55例ELISA双试剂与NAT同时反应性,确证阳性52例(94.5%),不确定3例(5.5%)。59例ELISA双试剂反应性标本中,万泰S/CO大于10确证阳性率98%;伯乐S/CO大于6确证阳性率100%;索林S/CO在1~6区间确证阳性率33%,S/CO在6~10之间确证阳性率91%,S/CO大于10确证阳性率(100%)。结论ELISA双试剂反应性、核酸反应性和确证阳性存在着极大的符合关系,且较高的S/CO值预示HIV确证阳性的可能性越大。
Objective To understand the situation of human immunodeficiency virus (HIV) infection in blood donors in Tianjin area,and to explore the correlation between ELISA test results and S/CO value,NAT screening results and confirmatory results.Methods Objective to analyze the HIV screening and confirmation data of blood donors from September 1,2016 to August 31,2017 in our laboratory.Results During the study period,292 of 157 130 voluntes were screened for reactivity,among them,59 cases of enzyme linked immunosorbent assay (ELISA) double reagent reactivity,of which,52 cases were confirmed positive (88%);among them,56 cases of Nucleic Acid Test (NAT) reactivity (including 1 case of window phase),of which,53 cases (94.6%) were confirmed positive;among 55 cases of double reagent reactivity of ELISA with NAT reactivity,52 cases (94.5%) were confirmed positive,3 cases were uncertain (5.5%).Among 59 cases of ELISA double reagent reactivity samples,the positive rate of the S/CO values greater than 10 was 98% duing using the Wantai reagent;the positive rate of the S/CO values greater than 6 was 100% duing using the Bole reagent;duing using the Suolin reagent,the positive rate of the S/CO values between 1~6 was 33%,the positive rate of the S/CO values between 6-10 was 91% and the positive rate of the S/CO values greater than 10 was 100%.Conclusion ELISA double reagent reactivity,nucleic acid reaction and confirmed positive exist with great relationship and the higher S/CO value predicts the greater possibility of HIV confirmed positive.
作者
王全慧
谢月娜
潘彤
WANG Quanhui;XIE Yuena;PAN Tong(Tianjin Blood Center laboratory,Tianjin 300110,china)
出处
《国际检验医学杂志》
CAS
2019年第A01期42-45,共4页
International Journal of Laboratory Medicine
