摘要
以杂种核桃组培苗的叶片和叶柄为材料,设置5种激素组合进行叶片和叶柄愈伤组织的诱导,并设置4种激素组合进行愈伤组织再分化的诱导,以研究杂种核桃的组培再生技术。结果表明:叶柄较叶片更容易发生愈伤组织,并且褐化率较低;TDZ 4mg·L^-1+2,4-D 1.5mg·L^-1和6-BA 0.5mg·L^-1+NAA 0.5mg·L^-1+TDZ1.0mg·L^-1 2种植物激素组合最适合愈伤组织的诱导。产生的愈伤组织呈白色和黄白色,为非胚性愈伤组织。在4种激素组合下愈伤组织器官,但均未诱导成功。部分愈伤组织有增殖,培养1个月后全部褐化。研究说明杂种核桃的愈伤组织较容易诱导发生,而器官的再分化较困难。
In order to study the tissue culture regeneration technology of hybrid walnut,in this study we used the leaves and petioles of hybrid walnut tissue culture seedlings as materials,and 5 hormone combinations were set to induce callus of leaves and petioles.In addition,4 hormone combinations were set to induce callus redifferentiation.The results showed that the petioles was more easy to induce callus than leaves,and the browning rate was lower.We found two hormone combinations which TDZ4 mg·L^-1+2,4-D1.5 mg·L^-1 and 6-BA0.5 mg·L^-1+NAA0.5 mg·L^-1+TDZ1.0 mg·L^-1 were the most suitable for callus induction.The callus induced was white and yellowish white,but it was non-embryonic callus.Organ induction from callus was all not successful under the combinations of 4 hormones.Some of the callus proliferated and all browned after cultured one month.It showed that callus of hybrid walnut was easy to induce,but the redifferentiation of organs was difficult.The results of this study laid a certain foundation for further study on walnut callus organ differentiation.
作者
戴小云
武春霞
Dai Xiaoyun(Tianjin Agricutual College,Tianjin 300380,China)
出处
《安徽农学通报》
2019年第13期44-47,共4页
Anhui Agricultural Science Bulletin
