摘要
为探讨干旱胁迫对猪苓( Polyporus umbellatus )菌丝生长与多糖物质积累的影响,采用不同质量浓度(0、50、100、150、200、250 g/L)PEG-6000处理,分析干旱胁迫下猪苓菌丝生长相关指标及多糖合成相关酶活性的变化。结果表明,100 g/L PEG-6000处理下,猪苓菌丝生物量、多糖合成相关酶活性及多糖质量分数最高,其中猪苓菌丝生物量、己糖激酶(HK)活性、磷酸葡萄糖异构酶(GPI)活性、α-磷酸葡萄糖变位酶(α-PGM)活性及多糖质量分数分别较对照组增加60.40%、27.88%、19.89%、28.73%和106.00%。当PEG-6000质量浓度>150 g/L时,猪苓菌丝生物量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、丙二醛(MDA)质量摩尔浓度、可溶性蛋白质量分数及多糖质量分数均随胁迫程度增大显著降低。因此,适度干旱胁迫有利于猪苓菌丝生长和多糖合成。
To explore the effect of the drought stress on the growth and the accumulation of polysaccharides of Polyporus umbellatus mycelium,with different mass concentrations of PEG-6000 (0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L and 250 g/L),the strain of P.umbellatus was treated by PEG-6000 to simulate drought stress. Through liquid culture, the growth index and enzyme activities related to polysaccharide synthesis of mycelium were evaluated. The results indicated that the biomass, enzyme activities and polysaccharide mass fraction of P.umbellatus were the highest in the concentration of PEG-6000 (100 g/L). Meanwhile, the biomass, HK activity, GPI activity,α-PGM activity and polysaccharide mass fraction significantly increased by 60.40%, 27.88%, 19.89%, 28.73%, 106.00%, respectively, compared to the CK group. When the mass concentration of PEG-6000 was exceeded 150 g/L, the biomass, POD activity, SOD activity, MDA molality, soluble proteins mass fraction and polysaccharide mass fraction of mycelium significantly decreased with the increase of drought stress. Therefore, moderate drought stress are conducive to growth and polysaccharide synthesis of P.umbellatus .
作者
徐梦馨
郭宏波
廉冬
马珍璐
张跃进
孙建华
XU Mengxin;GUO Hongbo;LIAN Dong;MA Zhenlu;ZHANG Yuejin;SUN Jianhua(College of Chemistry and Pharmacy, Northwest A&F University, Yangling Shaanxi 712100,China;College of Life Sciences, Northwest A&F University, Yangling Shaanxi 712100,China;Shaanxi Hanwang TCM Technology Co.Ltd.,Lueyang Shaanxi 724300,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2019年第7期1179-1186,共8页
Acta Agriculturae Boreali-occidentalis Sinica
基金
陕西省科技统筹创新工程计划(2016KTCL02-10)~~
关键词
猪苓
菌丝
PEG-6000
干旱胁迫
多糖合成
Polyporus umbellatus
Mycelium
PEG-6000
Drought stress
Polysaccharide synthesis
