摘要
目的:研究雌孕激素对牙周膜细胞hPDLCs的成骨化的影响,探讨雌孕激素是否有协同作用。方法:原代培养hPDLCs,取第4~6代细胞用于实验。分为空白对照组,孕酮组,雌二醇组,孕酮组和雌二醇共同处理组。用含有50nmol/L孕酮、50nmol/L雌二醇的培养液培养hPDLCs24h或48h后,采用MTT法检测在孕激素、雌激素作用下hPDLCs的增殖情况。通过Real-timePCR方法检测雌孕激素对牙周膜细胞hPDLCs中PLAP-1、OPG、ODF、Col-1和MMP-9等成骨分化相关基因表达的影响。茜素红染色定量分析雌孕激素对矿化hPDLCs细胞成骨分化的影响。结果:孕酮、雌激素干预hPDLCs后,细胞的增殖显著高于对照组(P<0.05),孕酮和雌激素共同处理组显著高于两者分别处理组(P<0.05)。与普通培养组相比较,孕酮和雌激素对成骨促进相关基因表达有显著的提高作用(P<0.05),两者共同作用促进相关基因更为明显。雌孕激素处理对矿化hPDLCs细胞成骨分化的影响高于对照组(P<0.05),两者联合显著高于单独处理组(P<0.01)。结论:孕酮和雌激素能够协同促进人牙周膜细胞的增殖和成骨分化。
Objective: To detect the proliferation and the expression of related genes of osteogenic differentiation in human periodontal ligament cells (hPDLCs) by estrogen and progesterone.Methods: The hPDLCs were isolated,cultured and stimulated with estrogen and/or progesterone for 24h or 72h.MTT assay was performed to assess the effects of estrogen and/or progesterone on cell proliferation.To quantify the effects of estrogen and/or progesterone on osteogenic differentiation of hPDLCs,real-time PCR was performed in estrogen and/or progesterone-treated hPDLCs.Results: Estrogen and/or progesterone could stimulate the proliferation of the hPDLCs,and promote the expression of PLAP-1,OPG,MGP,ODF,COL-1,and MMP-9 in hPDLCs.Furthermore,estrogen and/or progesterone exerted the synergistic effect in the promotion of cell proliferation and differentiation of hPDLCs.The effect of estrogen or progesterone treatment on osteoblast differentiation of mineralized hPDLCs cells was increased in contrast to the control group ( P <0.05).The combined treatment group was significantly higher than the single treatment group ( P <0.01).Conclusion: Estrogen and progesterone can synergistically stimulate the proliferation and differentiation of the hPDLCs.
作者
程海燕
沈兰花
张瑞
孟玲娜
刘迪
刑北昱
陶冠男
CHENG Haiyan;SHEN Lanhua;ZHANG Rui;MENG Lingna;LIU Di;XING Beiyu;TAO Guannan(University of Jiamusi,Henlongjiang Province,Jiamusi 154000;Department of Stomatology,Nangang Branch of Heilongjiang Provincial Hospital,Harbin 150001,China)
出处
《口腔医学研究》
CAS
北大核心
2019年第7期657-660,共4页
Journal of Oral Science Research
基金
黑龙江省自然科学基金(编号:H2016044)
关键词
孕酮
雌二醇
人牙周膜细胞
细胞增殖
成骨分化
estrogen
progesterone
human periodontal ligament cells
cell proliferation
differentiation
