摘要
目的:建立柱切换高效液相色谱法测定利培酮及其代谢物的血药浓度方法学。方法:采用柱切换法对利培酮及其代谢物血药浓度进行测定,并对利培酮及9-OH利培酮柱切换-HPLC方法学进行验证。结果:柱切换-HPLC法线性良好,利培酮(RIS)及其产物9-OH利培酮(9-OHRIS)线性范围均为4~160μg/L,方法回收率分别为88.3%~115.7%及81.9%~106.8%,日内RSD分别为1.29%~2.01%及1.62%~3.41%,日间RSD分别为2.19%~3.10%及2.02%~4.69%,重复性为2.05%。结论:柱切换-HPLC法能够快捷准确的测定临床样本,为病人提供更加准确及时的服务。
Objective: A method for determination of risperidone and its metabolites by column switching HPLC was established. Methods: The plasma concentration of risperidone and its metabolites was determined by column switching method,and verified the column switching-HPLC method of risperidone and 9-OH risperidone. Results: The Column Switching-HPLC method which used on RIS and 9-OHRIS have a linear range of 4 to 160μg/L,with recovery rates of 88.3 %~ 115.7 %and 81.9 %~ 106.8 %,respectively.The RSD during the day was 1.29 %~ 2.01 %and 1.62 %~ 3.41 %,and the RSD during the day was 2.19 %~ 3.10 %and 2.02 %~ 4.69 %,Repeatability is 2.05 %. Conclusion: The column switching-HPLC method can quickly and accurately determine clinical samples and provide patients with more accurate and timely services.
作者
仝利俊
马睿婷
王来兵
乔俊缠
TONG Li-jun;MA Rui-ting;WANG Lai-bing(Outpatient Department of Inner Mongolia National Center For Mental Health,Hohhot 010010 China)
出处
《内蒙古医科大学学报》
2019年第4期344-346,共3页
Journal of Inner Mongolia Medical University
基金
自治区卫生计生科研计划项目(201703075)
