摘要
采用组织培养方法对兰州百合鳞片不同部位芽诱导和试管小鳞茎生根膨大进行研究。结果表明,兰州百合最佳外植体为鳞片下部,其次为鳞片中部,鳞片上部不适于作外植体材料。组培小鳞茎一次膨大和二次膨大分别处理60、90、120d的鲜重和横径均随着处理时间的增加而增大,且二次膨大比一次膨大效果显著。
The method of tissue culture was used to study the bud induction and the rooting and enlargement of small tube bulbs in different parts of Lilium davidiivar.The results showed that the best explant of Lanzhou lily is the lower part,followed by the middle part and the upper part is not suitable for explant material.The fresh weight and transversal diameter of the small bulb in tissue culture increased with the increase of the treatment time at 60 d,90 d and 120 d,respectively.Moreover,the effect of secondary expansion is more significant than that of the primary expansion.
作者
裴怀弟
林玉红
李淑洁
厚毅清
叶春雷
张海林
PEI Huaidi;LIN Yuhong;LI Shujie;HOU Yiqing;YE Chunlei;ZHANG Hailin(Institute of Biotechnology,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Yuzhong Agricultural Technology Extension Center,Yuzhong Gansu 730100,China)
出处
《甘肃农业科技》
2019年第7期29-32,共4页
Gansu Agricultural Science and Technology
基金
甘肃省农业科学院中青年基金项目(2017GAAS94)
甘肃省农业科学院科技支撑计划(2017GAAS36)
甘肃省农业科学院中青年基金项目(2017GAAS92)
兰州市人才创新创业项目(2018-RC-130)
关键词
兰州百合
组织培养
诱导
小鳞茎
Lanzhou lily
Tissue culture
Induction
Small bulb
