摘要
分离纯化广西怪石滩海域海绵可培养共附生细菌,分析其多样性并研究共附生细菌发酵产物对甘蔗鞭黑粉菌的抑制活性,为研发新型生物农药提供参考依据。采用8种培养基分离纯化海绵共附生细菌,运用16S rDNA测序方法进行菌种鉴定及多样性分析,采用牛津杯法测试细菌发酵产物对甘蔗鞭黑粉菌的抑制效果。经形态学分析及16SrDNA比对,海绵中共获得34株共附生细菌,隶属于18科24属。活性检测结果表示,存在14株对甘蔗鞭黑粉菌有抑制效果的菌株。海绵中可培养共附生细菌物种多样性丰富,部分菌株的发酵产物对甘蔗鞭黑粉菌具有较强的抑制效果,有成为新型生物农药的潜力。
The purpose of this study was to investigate the distribution and the diversity of co-bacteria of Siphonochalina flexa isolated from Guaishi sea beach and to study its antifungal activities of the fermentation liquids against Sporisorium scitamineum.Eight different isolation media were used to isolate and purify the co-bacteria from S.flexa.The 16S rDNA was employed to explore the diversity of the isolated strains.The effects of the fermentation liqiuds against S.scitamineum were tested with the method of Oxford cup.After the morphological analysis and 16S rDNA alignment,34 strains of co-bacteria were isolated from S.flexa,belonging to 18 families and 24 genera.Through testing the antifungal activities,we screened 14 kinds of bacteria that had the inhibitory effect on S.scitamineum.The cultivated bacteria in S.flexa were rich in species and most of them showed strong activity of against S.scitamineum.
作者
江蕾
李蜜
李智
易湘茜
高程海
周桂
JIANG Lei;LI Mi;LI Zhi;YI Xiangxi;GAO Chenghai;ZHOU Gui(Institute of Marine Medicine / Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200,China;School of Marine Sciences and Biotechnology,Guangxi University of Nationalities / Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and Modifications / Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources,Nanning,Guangxi 530006,China)
出处
《热带作物学报》
CSCD
北大核心
2019年第7期1421-1427,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.41566004)
广西自然科学基金项目(No.2016GXNSFBA380095)
广西中医药大学岐黄工程高层次人才团队培育项目(No.2018006)
关键词
海绵
共附生细菌
物种多样性
甘蔗鞭黑粉菌
抑菌活性
Siphonochalina flexa
co-bacteria
species diversity
Sporisorium scitamineum
antifungal activities
