摘要
目的比较观察老年C57BL/6J小鼠与年轻C57BL/6J小鼠外周血及免疫系统相关指标的差异。方法19月龄和2月龄的C57BL/6J小鼠分别取材检测外周血细胞计数、免疫脏器指数和外周血免疫细胞分型。结果与年轻小鼠比较,老年小鼠外周血中红细胞和血小板计数升高,中性粒细胞比例升高,淋巴细胞比例下降(P<0.001),CD4^+细胞比例明显降低(P<0.001),胸腺指数明显降低(P<0.001)。结论老年小鼠血细胞发生分化偏移,辅助性T细胞数量下降,与年轻小鼠有明显差异,与人造血免疫老龄化改变类似。本项研究为免疫衰老相关问题研究提供基础数据。
Objective To investigate the changes of peripheral blood and immune cell phenotypes between aged and young C57BL/6J mice.Methods Peripheral blood cell count,immune organ coefficient and peripheral blood immune cell phenotype in aged C57BL/6J mice(19 months)and 2-month-old C57BL/6J mice used as control adult mice were analyzed.Results Compared with young mice,the aged mice had an increased peripheral blood platelet count,increased proportion of neutrophils,and a decreased proportion of lymphocytes(P<0.001).The thymus coefficient was significantly reduced(P<0.001).The percentage of CD4^+cells in the peripheral blood immune cell typing was obviously reduced(P<0.001).Conclusions The blood cells in aged mice are differentially skewed,and helper T-cell numbers declined.The changes are similar to those in the aging human.This study provides basic data for research on immuno-senescence.
作者
管博文
卢延华
苏路路
王玉全
李德冠
孟爱民
GUAN Bowen;LU Yanhua;SU Lulu;WANG Yuquan;LI Deguan;MENG Aimin(Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases,Beijing 100021,China;Institute of Radiation Medicine,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine,Tianjin 300192)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第7期24-28,共5页
Chinese Journal of Comparative Medicine
基金
中国医学科学院医学与健康科技创新工程经费资助(2017-I2M-3-015)
国家重点研发计划(2017YFA0105204)
国家自然科学基金(81573094)
