摘要
目的研究急性运动对大鼠心肌miRNA-30a及其对自噬相关基因表达的影响,探讨miRNA-30a对运动后不同恢复时期心肌自噬活性的调控机制。方法SD大鼠随机分为安静对照组(Con),运动后0 h组(E0),运动后3 h组(E3)和运动后12 h组(E12)。急性运动后按不同时间点取材。使用实时荧光定量聚合酶链反应(PCR)检测左心室肌中ACE mRNA、miRNA-30a、Beclin-1 mRNA和LC3 mRNA表达量。结果与安静对照组相比,运动后0 h组左心室肌中ACE mRNA和miRNA-30a表达显著性升高(P<0.05),Beclin-1 mRNA和LC3 mRNA表达也显著提高(P<0.05),在运动后3 h均恢复到安静水平。结论急性运动可以显著提高心肌ACE mRNA、miRNA-30a、Beclin-1 mRNA和LC3 mRNA表达,这种一过性升高可能是由运动应激导致的。
Objective To study the influence of acute exercise on rat myocardial microRNA-30a and autophagy-related gene expression,and investigate the possible mechanism through which microRNA-30a regulates myocardial autophagy during different recovery periods after exercise.Methods SD rats were randomly divided into a control group(Con),a 0h after exercise group(E0),a 3h after exercise group(E3),and a 12 h after exercise group(E12).Real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of ACE mRNA,microRNA-30a,Beclin-1 mRNA,and LC3 mRNA in the left ventricular myocardium.Results Compared with the control group,in the E0 group,ACE mRNA and microRNA-30a gene expression in the left ventricular muscle was significantly increased(P<0.05),and Beclin-1 mRNA and LC3 mRNA expression also significantly increased(P<0.05).This was recovered to resting state approximately 3 h after exercise.Conclusions Acute exercise can significantly improve myocardial ACE mRNA,microRNA-30a,Beclin-1 mRNA and LC3 mRNA expression.This transient increase may be caused by exercise-induced stress.
作者
杨赟
王智强
王蕴红
YANG Yun;WANG Zhiqiang;WANG Yunhong(Department of Postgraduates,Capital University of Physical Education and Sports,Beijing 100191,China;School of Sport Science and Health,Capital University of Physical Education and Sports,Beijing 100191)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第7期76-79,共4页
Chinese Journal of Comparative Medicine
基金
北京市学科建设项目分子生物学研究平台(15516014)
