摘要
目的 建立尿中甲乙酮的顶空气相色谱测定方法.方法 尿液样品中甲乙酮经过顶空技术预处理后,取一定量的顶空气体注入气相色谱仪,经过毛细管柱分离,氢火焰离子化检测器检测,保留时间定性,峰高或峰面积定量.结果 该方法尿中甲乙酮浓度在0.01~6.0 μg/ml呈线性,回归方程y=13.316x+0.849 7,r=0.999 7;最低检出浓度0.01 μg/ml,方法回收率为96.0%~100.4%;日内、日间相对标准偏差(RSD)分别为2.2%~5.5%和2.5%~6.1%,尿样在4℃冰箱中可保存21 d.结论 该方法的灵敏度高、准确度好,操作步骤简便,适用于尿中甲乙酮的检测.
Objective To establish a method for determination of methyl ethyl ketone in urine by headspace gas chromatography. Methods In the urine sample﹙hereinafter referred to as urine sample﹚, methyl ethyl ketone is pretreated by headspace technology, and a certain amount of head air is injected into the gas chromatograph, separated by capillary column, detected by hydrogen flame ionization detector, and the retention time is qualitative and the peak height is high. Peak area. Results Good linearity was in the range of 0.01 to 6.0 μg/ml with a regression equation of y=13.316x+0.8497 and γ=0.9997.The minimum detectable concentration of methyl ethyl ketone was 0.01 μg/ml. The range intra-day RSD and inter-day RSD were 2.2%-5.5% and 2.5%-6.1% respectively. Urine samples can be stored for 20 days in the refrigerator at 4 ℃. Conclusion The method has a high advantage of sensitivity and accuracy, and also easy to operate. Therefore, it is suitable for the determination of methyl ethyl ketone in urine.
作者
张梦萍
于志刚
孙亚昕
王翠娟
刘仲
卫海燕
邹薇
Zhcuig Mengping;Yu Zhigang;Sun Yaxin;Wang Cuijuan;Liu Zhong;Wei Haiyan;Zou Wei(Shandong A cade my of Occupational Health and Occupational Medicine , Shandong First Medical University & Shandong A cade my of Medical Sciences , Jinan 250062, China;Jinan Center For Disease Control and Prevention, Jinan 250012, China)
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2019年第6期457-459,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
关键词
甲乙酮
尿
顶空萃取
色谱法
Methyl ethyl ketone
Urine
Headspace
Chromatography
