摘要
目的了解毒力基因pagC与msgA在福建省分离率较高的4种常见血清型沙门菌中的分布差异特征,为福建省沙门菌监测提供科学的实验数据,为进一步有效防控沙门菌病提供依据。方法选取福建省2010年以来分离留存的鼠伤寒沙门菌、肠炎沙门菌、德尔卑沙门菌及斯坦利沙门菌共166株,通过常规PCR进行pagC和msgA基因的检测。采用SPSS 17.0进行数据统计。结果 166株沙门菌中有68株携带毒力基因pagC,总检出率为40.96%;93株携带毒力基因msgA,总检出率为56.02%。毒力基因pagC与msgA在鼠伤寒、肠炎、德尔卑和斯坦利4种血清型沙门菌分离株中的携带率差异均有统计学意义(χ^2pagC=90.22、χ^2msgA=10.33,P<0.01)。沙门菌有4种毒力基因携带模式:VP1:pagC+msgA-(4.85%)、VP2:pagC-msgA+(20.00%)、VP3:pagC+msgA+(36.36%)和VP4:pagC-msgA-(38.79%),后两种为优势基因携带模式。结论福建省沙门菌中均检出一定比例的毒力基因pagC与msgA。这2个毒力基因的分布存在血清型差异。而且这2个毒力基因携带模式呈多态性分布特征。
Objective To understand the virulence gene pagC and msgA in the 4 common serotypes of Salmonella enteric in Fujian province,so as to provide scientific experimental data for salmonella monitoring,and provide the basis for the further effective prevention and control of salmonellosis. Methods A total of 166 strains of Salmonella enterica isolated including 4 serotypes of S. typhimus^S. enteritisDel humiliation and S. Stanley . from Fujian province since 2010 were selected. The conventional PCR method was used to amplify the virulence gene pagC and msgA. SPSS 17 was used for data statistics. Results Virulence gene pagC was detected in 68 of the 166 strains of salmonella, and the detection rate was 40. 96%. Virulence gene msgA was detected in 93 of the 166 strains of salmonellathe detection rate was 56. 02%.There was significant difference in the detection rate of pagC and msgA in the 4 serotypes of S. typhimus, S. enteritis, S. Del humiliation and S. Stanley (χ^2pagC=90.22、χ^2msgA=10.329, P <0. 01). There were 4 kinds of patterns of gene delivery in salmonella: VP1: pagC+msgA-(4. 85%), VP2: pagC-msgA +(20. 00%), VP3 : pagC+msgA+(36. 36%) and VP4:pagC-msgA-(38. 79%). The latter 2 were the most common patterns of gene delivery. Conclusion A certain proportion of pagC and msgA are detected in Salmonella isolates from Fujian province. The distribution of these 2 virulence genes is different from serotypes. And the patterns of these 2 virulence genes delivery present polymorphism distribution characteristics.
作者
黄梦颖
陈建辉
邱玉锋
杨劲松
谢方钦
李曲文
HUANG Meng-ying;CHEN Jian-hui;QIU Yu-feng;YANG Jin-song;Xie Fang-qin;Li Qu-wen(Fujian Center for Disease Control and Prevention ,Fuzkou,Fujian,350001,China)
出处
《预防医学论坛》
2019年第6期414-416,420,共4页
Preventive Medicine Tribune
基金
福建省卫生和计划生育委员会青年科研课题(项目号:2014-1-26)
福建省科技厅面上项目(项目号:2019J01093)
关键词
沙门菌
毒力基因
血清型
Salmonella
Virulence genes
Serotype
