摘要
丝状真菌米曲霉是发酵工业中的重要菌种,常用于酱油、豆豉、次级代谢产物及酶类产品的生产。近年来,随着米曲霉全基因组序列的破译,米曲霉的功能基因组研究成为一个热点。由于米曲霉转化体系的不成熟和多核现象的存在,导致了米曲霉功能基因组研究的进展缓慢。综述了米曲霉目前所用的几种遗传转化策略,并比较了它们之间的优缺点;介绍了近几年米曲霉的功能基因组研究进展,重点阐述了米曲霉蛋白质分泌、分生孢子形成和次级代谢产物合成等重要的功能基因研究结果;最后对米曲霉在传统酿造和工业用酶及其次级代谢产物生产的应用上进行了展望。
Aspergillus oryzae,a filamentous fungus,is an important strain in fermentation industry and is often used in the production of soy sauce,Douchi,secondary metabolites and enzyme-like products. In recent years,with the decoding of whole genome sequence of A. oryzae,the functional genomics of A. oryzae has become a hot topic. Due to the immaturity of A. oryzae transformation system and the existence of multinucleation,the research progress of functional genome on A. oryzae is slow. This paper first reviews the genetic transformation strategies of A. oryzae,as well as compares their advantages and disadvantages. Then the paper introduces the research progress of functional genomics of A. oryzae in recent years,mainly including protein secretion,conidia formation and secondary metabolite synthesis of A. oryzae. Finally,the application of A. oryzae in the production of enzymes and secondary metabolites is prospected.
作者
吴琴琴
孙敏
陈雨
付雅琴
曾斌
贺斌
WU Qin-qin;SUN Min;CHEN Yu;FU Ya-qin;ZENG Bin;HE Bin(College of Life Sciences,Jiangxi Science & Technology Normal University,Nanchang 330013)
出处
《生物技术通报》
CAS
CSCD
北大核心
2019年第8期186-192,共7页
Biotechnology Bulletin
基金
国家自然科学基金项目(31700068)
江西省教育厅一般项目(GJJ180605)
江西科技师范大学教改课题(JGYB-177816)
关键词
米曲霉
功能基因组
分生孢子
蛋白分泌
次级代谢产物
Aspergillus Oryzae
functional genomics
conidium
protein secretion
secondary metabolite
