摘要
为了探究榆树雄性不育系花芽形态的特征及内源激素在榆树雄性不育系花芽中的调控作用,在花芽发育的不同时期,观察白榆D33雄性不育株和可育株Y3、Y4的花芽形态特征,并利用石蜡切片对其花药的内部形态进行观察;采用高效液相色谱法,对不育植株和可育植株花芽发育过程的玉米素(ZT)、赤霉素(GA3)和生长素(IAA)含量的动态变化进行比较分析。结果表明,与可育株Y3、Y4相比,不育系白榆D33在花蕾发育期间,花丝不伸长,花药干瘪,不能形成正常的花粉,但其雌蕊发育正常,能接受花粉而形成翅果;不育系白榆D33的ZT含量明显高于Y3、Y4,GA3含量动态变化较大,IAA含量出现下降趋势,并且在D33中GA3/IAA和GA3/ZT值动态变化幅度较大。不育系白榆花芽雄蕊发育不正常,花药不能产生花粉进行自花授粉,但其雌蕊发育正常;不育系白榆花芽的ZT、GA3和IAA含量不稳定。
In order to explore the characteristics of flower bud morphology and the regulation of endogenous hormones in flower buds of eucalyptus male sterile lines,during different periods of flower bud development,the morphological characteristics of flower buds were observed,and the internal morphology of anthers was observed through paraffin section.High performance liquid chromatography (HPLC) was used to compare and analyze the content dynamic changes of ZT,GA 3,IAA between sterile plants and fertile plants.The results indicated that compared with fertile Y3 and Y4,during flower bud development of male sterility D33 the filaments were not elongated,the anthers were dry and could not form normal pollen,but their pistils developed normally and could receive pollen to form samara.The ZT content of the male sterility D33 was significantly higher than Y3 and Y4.The GA 3 content changed dynamically,and the IAA content showed a downward trend,and the dynamic changes of GA 3/IAA and GA 3/ZT values were high in D33.It was concluded that sterile buds of white buds were not normal.The anthers could not produce pollen for self-pollination,but their pistils developed normally.The contents of ZT,GA 3 and IAA in sterile buds were not stable.
作者
王晓叶
王力源
刘兴菊
贾泽云
杨春弟
梁海永
WANG Xiao-ye;WANG Li-yuan;LIU Xing-ju;JIA Ze-yun;YANG Chun-di;LIANG Hai-yong(College of Forestry,Agricultural Univeristy of Hebei,Baoding 071000,Hebei,China;Forest Resources Innovation and Protection Laboratory of Hebei,Baoding 071000,Hebei,China)
出处
《西北林学院学报》
CSCD
北大核心
2019年第4期76-81,90,共7页
Journal of Northwest Forestry University
基金
河北省科技计划项目“利用白榆雄性不育系进行榆树种质创新研究”(7226312D)
河北省科技计划项目“河北省重要乡土树种种质资源创列与利用技术研究(16226309D)”
关键词
白榆D33
雄性不育
花芽形态
内源激素
动态变化
Ulmus pumila D33
male sterility
flower bud morphology
endogenous hormone
dynamic change
