摘要
目的建立一种中空纤维离心超滤-高效液相色谱(HPLC)法分析人血浆中阿莫西林的浓度,并用于阿莫西林的药代动力学研究。方法血浆样本经中空纤维离心超滤技术预处理后,取超滤液20μL直接进行HPLC分析,用Hypersil BDS C18柱(150 mm×4. 6 mm,5μm),流动相:0. 05 mol·L^-1磷酸二氢钾(2 mol·L^-1KOH将pH调至5. 0)-乙腈=97. 5∶2. 5;流速:1. 0 mL·min^-1;柱温:30℃;检测波长:220 nm。结果人血浆中阿莫西林在0. 10~20. 3μg·mL^-1线性关系良好(r2=0. 999),检测线为2. 54×10^-2μg·mL^-1,方法回收率为91. 0%~100. 6%,绝对回收率为83. 1%~86. 8%,批内、批间RSD均不大于2. 9%,在室温放置8h、冻融循环3次和冰冻21 d的稳定性良好。成功用于我院人血浆中阿莫西林的药代动力学分析。结论本研究建立的方法简便、准确、灵敏,适用于人血浆中阿莫西林浓度分析。
Objective To establish a hollow fiber centrifugal ultrafiltration-high performance liquid chromatography( HPLC) method for the analysis of concentration of amoxicillin in human plasma and its application in pharmacokinetic study. Methods The plasma samples were prepared by hollow fiber centrifugal ultrafiltration,and filtrate 20 μL was directly injected into the HPLC for analysis. Hypersil BDSC18 column( 150 mm × 4. 6 mm,5 μm) was used. The mobile phase consisted of acetonitrile( 2. 5%) and 50 mmol·L^-1 potassium dihydrogen phosphate( 2 mol·L^-1 KOH adjusted to pH 5. 0,97. 5%) at the flow rate of 1. 0 mL·min^-1. The column temperature was maintained at 30 ℃. The detected wavelength was 220 nm. Results The good linear relationship were both obtained between the free concentration of amoxicillin from 0. 10-20. 3 μg·mL^-1( r2= 0. 999). The limit of detection for the analysis of free amoxicillin was 2. 54 × 10^-2μg·mL^-1. The method recovery rates of free amoxicillin were 91. 0%-100. 6%. The absolute recovery rates were 83. 1%-86. 8%,respectively. The intra-day and inter-day precision( RSD) were all less than 2. 9%. The amoxicillin was all stable at the conditions of maintaining at room temperature for 8 h,after freeze-thaw cycle and keeping at -80 ℃ for 21 d. The method was successfully applicated in amoxicillin pharmacokinetic study in our hospital. Conclusion The present method is simple,accurate and sensitive,which is suitable for the analysis of amoxicillin concentration in human plasma.
作者
董维冲
郭家良
李德强
刘姣
蒋晔
张志清
DONG Wei-chong;GUO Jia-liang;LI De-qiang;LIU Jiao;JIANG Ye;ZHANG Zhi-qing(Department of Pharmacy, The Second Hospital of Hebei Medical University,Shijiazhuang 050051,Hebei Province, China;Department of Orthopaedics,The Third Hospital of Hebei Medical University,Shijiazhuang 050000, Hebei Province,China;School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2019年第14期1497-1500,共4页
The Chinese Journal of Clinical Pharmacology
基金
2019河北省科技厅科技计划基金资助项目(19277728D)
关键词
阿莫西林
中空纤维
离心超滤
高效液相色谱
药代动力学
amoxicillin
hollow fiber
centrifugal ultrafiltration
high performance liquid chromatography
pharmacokinetic
