摘要
为研究不同形态的铁对剩余污泥水热反应过程中四环素抗性基因的削减影响,选取Fe^2+、Fe^3+和还原铁粉作为目标物,采用荧光定量PCR检测五种TC-ARGs(tetA、tetC、tetG、tetM和tetX)、16S rRNA及intI1 7种基因在水热反应前后的基因丰度变化。结果表明Fe能促进ARGs及intI1在水热反应过程中的削减,但不同形态Fe的促进效果不同,其中Fe 2+和Fe 3+的促进效果较还原铁粉明显,与空白组相比降低0.038~0.743个数量级。其中tetA的促进效果最为明显,可以多削减0.566个数量级。ARGs的相对丰度会在水热后增加,但Fe^2+的增加幅度最小,仅比反应前增加3.91E-04~1.07E-03。通过对intI1的检测发现,与TC-ARGs规律一致,Fe^2+能明显促进其削减及控制相对丰度的增加,综合考虑ARGs及intI1的绝对丰度和相对丰度变化,Fe^2+对水热反应中ARGs削减及控制作用最为明显。
In order to study the influence of different forms of ion on tetracycline resistance genes reduction during hydrothermal process of excess sludge,Fe^2+,Fe^3+ and reduced ion power were selected as objectives,and the abundance of TC-ARGs(tetA,tetC,tetG,tetM and tetX),16S rRNA and intI1 before and after hydrothermal process was detected by realtime fluorescence quantitative PCR (RTFQ PCR).The results showed that different forms of ion promoted the reduction of ARGs and intI1 in different degrees.The gene abundance treated by Fe^2+ and Fe^3+ reduced more obviously than that treated by reduction ion power,and was 0.038~0.743 order of magnitude lower than the blank group.The abundance of tetA was influenced most significantly,which reduced 0.566 order of magnitude more than that of other genes.The relative abundance of ARGs increased after hydrothermal process.The increase of relative abundance was the smallest when treated by Fe^2+,which was only 3.91E-04 ~ 1.07E-03.The detection of intI1 indicated that Fe^2+ could promote significantly the reduction of intI1 resistance genes and increasing of its relative abundance was the same as TC-ARGs.Considering the change of absolute abundance and relative abundance of ARGs and intI1,it was concluded that Fe^2+ had the most obvious effect on the reduction and controlling of ARGs among all the forms of ion.
作者
孙敏
陈红
SUN Min;CHEN Hong(College of Environmental Science and Engineering,Donghua University,Shanghai 201620,China)
出处
《能源环境保护》
2019年第4期4-10,共7页
Energy Environmental Protection
基金
国家自然科学基金(51878136)
江苏省自然科学基金(BK20160431)
