摘要
目的研究乳腺癌患者外周血CD62P和CA153的表达水平,并探讨其在乳腺癌诊断中的价值。方法采用流式细胞术(FCM)检测60例乳腺癌患者(乳腺癌组)、52例乳腺纤维瘤患者(纤维瘤组)和25例女性健康体检者(对照组)外周血CD62P比例,同时采用电化学发光法(ECLIA)检测血清中CA153含量,分析二者与乳腺癌患者临床病理因素的关系,应用受试者工作特征(ROC)曲线分析血清CD62P、CA153及二者联合检测对乳腺癌诊断的灵敏度和特异度。结果⑴乳腺癌组CD62P和CA153表达水平明显高于纤维瘤组及对照组,差异有统计学意义(均为P <0. 05),纤维瘤组与对照组之间差异无统计学意义(均为P> 0. 05)。⑵乳腺癌组外周血CA153、CD62P的表达水平均与其临床分期、淋巴转移有关(均为P <0. 05),两者表达呈正相关(r=0. 514,P <0. 05)。⑶CD62P、CA153联合检测对乳腺癌诊断的灵敏度和特异度均高于单项检测。结论乳腺癌患者外周血中CD62P呈高表达,其可能成为乳腺癌诊断的新标志物。
Objective To study the expression of CD62P and carbohydrate antigen 153 ( CA153) in peripheral blood of patients with breast cancer, and to explore the value of CD62P and CA153 in the diagnosis of breast cancer. Methods The CD62P ratio in peripheral blood of 60 patients with breast cancer (breast cancer group), 52 patients with breast fibroma ( fibroma group) and 25 healthy volunteers ( control group) was detected by flow cytometry ( FCM). The serum CA153 level was detected by electro chemiluminescence (ECLIA), and the relationship between their expression levels and c 1 inicopathological factors of breast cancer patients was analyzed. The sensitivity and specificity of serum CD62P, CA153 and their combination in the diagnosis of breast cancer were analyzed by receiver operating characteristic ( ROC) curve. Results (1) The expression levels of CD62P and CA153 in breast cancer group were significantly higher than those in fibroma group and control group ( P < 0. 05), but with no significant difference between fibroma group and control group ( P >0. 05).(2) The expression levels of CA153 and CD62P in peripheral blood of breast cancer group were correlated with clinical stage and lymphatic metastasis ( all P < 0. 05 ), and the expression levels of CA153 and CD62P were positively correlated ( r = 0. 514, P <0. 05).(3) The sensitivity and specificity of combined detection of CD62P and CA153 for breast cancer were higher than those of single detection. Conclusions CD62P is highly expressed in peripheral blood of patients with breast cancer, which may be a new marker for the diagnosis of breast cancer.
作者
赵树强
郭建平
曹荣
郭谦
李晓颜
李淑勤
Zhao Shuqiang;Guo Jianping;Cao Rong;Guo Qian;Li Xiaoyan;Li Shuqin(Department of Nuclear Medicine, Affiliated Fenyang Hospital of Shanxi Medical University, Fenyang032200, China;Department of General Surgery, Affiliated Fenyang Hospital of Shanxi Medical University yFenyang 032200, China;Department of Laboratory, Affiliated Fenyang Hospital of Shanxi Medical University,Fenyang 032200, China;Fenyang College of Shanxi Medical Universtity, Fenyang 032200, China)
出处
《中国医师杂志》
CAS
2019年第7期1011-1014,共4页
Journal of Chinese Physician
基金
吕梁市科技攻关项目(2016sfgg066-7)~~
关键词
乳腺肿瘤
流式细胞术
P选择素
抗原
肿瘤
Breast neoplasms
Flow cytometry
P-selectin
Antigens, neoplasm
