摘要
目的探讨肝细胞生长因子(hepatocyte growth factor,HGF)联合肌腱干细胞(tendon-derived stem cells,TDSCs)对大鼠内侧副韧带损伤模型的作用及相关机制。方法分离提取大鼠内侧副韧带源性肌腱干细胞并进行干细胞鉴定。实验动物分为四组,分别是对照组、HGF组、TDSCs组和HGF+TDSCs组,利用HGF和(或)TDSCs治疗大鼠内侧副韧带损伤模型。4周后,分别从大体形态、免疫组织化学及生物力学角度对治疗效果进行评价。结果各治疗组在大体观察和形态学观察明显促进大鼠内侧副韧带的损伤愈合,HGF+TDSCs组效果更加显著。生物力学方面,各治疗组在愈合肌腱的最大负荷比较无统计学意义。结论联合应用HGF和TDSCs治疗大鼠内侧副韧带损伤,更有利于肌腱组织形态学的优质愈合,为肌腱损伤提供一个新的治疗策略。
Objective To investigate the effect and the mechanism of hepatocyte growth factor ( HGF) combined with tendon-derived stem cells ( TDSCs) on the medial collateral ligament injury model of rats. Methods The rat medial collateral ligament-derived tendon stem cells were isolated and identified. The experiment animals were divided into four groups: control group,HGF group,TDSCs group and HGF + TDSCs group. Rat MCL injury was treated by HGF and /or TDSCs. Response to the cell therapy was assessed by gross observation,histological evaluation and biomechanical testing at 4 weeks after each treatment. Results Although each cell therapy group included macroscopic and morphological recovery in healing MCLs,the combined use of HGF /TDSCs led to further improvement in healing quality. Biomechanical testing showed that the failure load of the healing ligament had no statistically significant difference between the treatment groups. Conclusion The combination of HGF and TDSCs as a cell therapy thus enhances healing function towards normal after MCL injury,which represents a promising strategy for ligament injury.
作者
杨书龙
郭铁云
张泽楠
陆文君
韩沛霖
李昭铸
YANG Shu-long;GUO Tie-yun;ZHANG Ze-nan;LU Wen-jun;HAN Pei-lin;LI Zhao-zhu(Department of Pediatric Surgery,The Second Affiliated Hospital of Harbin Medical University;Basic Medical College,Harbin Medical University,Harbin 150081,China)
出处
《哈尔滨医科大学学报》
CAS
2019年第2期111-115,共5页
Journal of Harbin Medical University
基金
国家自然科学基金资助项目(81871837
81572117)
黑龙江省卫生计生委科研课题(2014-341
2016-161)
黑龙江省博士后资助经费(LBH-Z15128)
哈尔滨医科大学附属第二医院中青年创新科学研究基金(CX2016-04)
关键词
肝细胞生长因子
肌腱干细胞
大鼠
内侧副韧带
韧带损伤
hepatocyte growth factor
tendon-derived stem cells
rat
medial collateral ligament
ligament injury
