摘要
目的:探讨三种方法检测幽门螺杆菌(Hp)在临床试验中的应用价值。方法:收集98例临床血清样本,运用Hp抗体检测试验(金标法)、尿素14C呼气试验及幽门螺杆菌抗体检测(胶乳免疫比浊法)三种检测方法检测Hp。结果:Hp抗体检测试验(金标法)、尿素14C呼气试验、胶乳免疫比浊法阳性检出率分别为28.57%、42.86%和35.71%。以尿素14C呼气试验的检测为基准,金标法、胶乳免疫比浊法特异度100%、75.7%,灵敏度66.67%、51.4%;尿素14C呼气试验、胶乳免疫比浊法阳性符合率为66.7%、51.4%,阴性符合率为100%、84.1%,尿素14C呼气试验、胶乳免疫比浊阳性符合率、阴性符合率与金标准比较有显著性差异(P<0.05);胶乳免疫比浊法阳性符合率为33.3%,阴性符合率为62.5%,假阳性和假阴性率高,与尿素14C呼气试验比较无显著性差异(P>0.05)。结论:胶乳免疫比浊法敏感性较差,尚不适合临床使用,其方法学还有待改进,尿素14C呼气试验和金标法检测Hp各有利弊,在临床运用中可结合自身和患者的实际情况选择检测方法,以提高检出率。
Objective: To evaluate the application value of three methods for detection of Helicobacter pylori(Hp) in the clinical screening tests.Methods: 98 serum samples were collected and analyzed helicobacter pylori by helicobacter pylori antibody test(gold immnnochromatography), latex immunoturbidimetric and 14 C urea breath tests. Results: In the 98 serum samples, the positive rate of helicobacter pylori antibody test(gold immnnochromatography)was 28.57%, the positive rate of 14 C urea breath test was 42.86%, the positive rate of latex immunoturbidimetric was 35.71%.Based on the detection of urea 14C urea breath test, the specificity of gold immnnochromatography was 100%, and the sensitivity was 66.67%, the specificity of latex immunoturbidimetric method was 75.7%, and the sensitivity was 51.4%. The positive coincident rate of 14 C urea breath test with gold immnnochromatography was 66.7%, the negative coincident rate was 100%, there were statistical significance(P<0.05);the positive coincident rate of gold immnnochromatography with latex immunoturbidimetric was 51.4%, the negative coincident rate was 84.1%, there were statistical significance(P<0.05). The positive coincident rate of 14 C urea breath test with latex immunoturbidimetric was 33.3%, the negative coincident rate was62.5%, the false positive and false negatives were high, there were not statistical significance(P>0.05). Conclusions: Latex immunoturbidimetric had poor sensitivity was not suitable for clinical use, its methodology need to be improved. The detection of Hp with 14 C urea breath test and gold immnnochromatography were used to detect had its own pros and cons in clinical application, the detection method should be selected based on the actual situation of the patient and oneself to improve the detection rate.
作者
霍咏姬
陈超
钟雪芬
HUO Yong-ji;CHEN-Chao;ZHONG-Xuefen(Huangpu Hospital of Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou,Guangdong 510310)
出处
《按摩与康复医学》
2019年第16期38-40,共3页
Chinese Manipulation and Rehabilitation Medicine
关键词
幽门螺杆菌
尿素14C呼气试验
金标法
胶乳免疫比浊法
Helicobacter pylori
14C urea breath test
gold immnnochromatography
latex immunoturbidimetric
