摘要
目的:探究酪氨酸蛋白激酶-7(PTK7)、人表皮生长因子受体2(HER-2)、微小染色体维持蛋白5(Mcm5)蛋白在食管鳞癌(ESCC)组织中的表达水平及其临床预后价值。方法:选择180例ESCC患者作为研究对象,采集其肿瘤组织,并随机收集100例癌旁组织,通过免疫组化染色检测PTK7、HER-2、Mcm5蛋白的表达水平,以及其与临床病理特点以及与预后的关系。结果:ESCC组织中PTK7、HER-2、Mcm5的阳性率均显著高于癌旁组织(P<0.05)。出现淋巴转移组织中PTK7的阳性率显著高于未出现淋巴转移(P<0.05)。瘤体直径大于2 cm、TNM分期为Ⅲ期和Ⅳ期以及合并淋巴转移ESCC组织HER-2阳性率显著升高(P<0.05)。TNM分期为Ⅲ期和Ⅳ期以及合并淋巴转移ESCC组织Mcm5阳性率更高(P<0.05)。ESCC组织中PTK7表达水平与淋巴转移正相关(P<0.05),HER-2与TNM分期和淋巴转移正相关(P<0.05),Mcm5与瘤体大小、TNM分期和淋巴转移正相关(P<0.05)。PTK7、HER-2、Mcm5阳性的ESCC患者的无瘤生存期显著短于阴性组(P<0.05)。结论:在ESCC组织中PTK7、HER-2、Mcm5的表达水平均显著上调,并且高水平的HER-2、Mcm5预示着更严重的病理分期以及淋巴转移,并且PTK7、HER-2、Mcm5与更差的预后有关。
Objective: To investigate the expression levels and clinical prognosis of tyrosine protein kinase-7(PTK7), human epidermal growth factor receptor 2(HER-2) and minichromosome maintenance protein 5(Mcm5) in esophageal squamous cell carcinoma(ESCC) value. Methods: A total of 180 patients with ESCC were enrolled as the study subjects. Tumor tissues were collected and 100 paracancerous tissues were randomly collected. The expression levels of PTK7, HER-2 and Mcm5 proteins were detected by immunohistochemical staining, and the relationship with clinicopathological characteristics and prognosis was also measured. Results:: The positive rates of PTK7, HER-2 and Mcm5 in ESCC tissues were significantly higher than those in adjacent tissues(P<0.05). The positive rate of PTK7 in lymphatic metastasis was significantly higher than that in lymphatic metastasis(P<0.05). The tumor body diameter was greater than 2 cm;the TNM stage was stage III and IV, and the HER-2 positive rate of ESCC tissues with lymphatic metastasis was significantly increased(P<0.05). The positive rate of Mcm5 in stage III and IV and combined lymphatic metastasis ESCC was higher that in TNM stage(P<0.05). The expression level of PTK7 in ESCC tissues was positively correlated with lymphatic metastasis(P<0.05). HER-2 was positively correlated with TNM stage and lymphatic metastasis(P<0.05). Mcm5 was positively correlated with tumor size, TNM stage and lymphatic metastasis(P<0.05). The tumor-free survival of PTK7, HER-2, and Mcm5-positive ESCC patients was significantly shorter than that of the negative group(P<0.05). Conclusion: The expression levels of PTK7, HER-2, and Mcm5 are significantly up-regulated in ESCC tissues;high levels of HER-2 and Mcm5 predict more severe pathological stage and lymphatic metastasis;PTK7, HER-2, Mcm5 are related with worse prognosis.
作者
易诗琼
宋燕
胡欣
张欣平
赵丽
YI Shi-qiong;SONG Yan;HU Xin;ZHANG Xin-ping;ZHAO Li(Second Clinical Medical College of North Sichuan Medical College, Nanchong Central Hospital Tumor Center, Nanchong 637000, Sichuan, China)
出处
《海南医学院学报》
CAS
2019年第15期1141-1144,1151,共5页
Journal of Hainan Medical University
基金
南充市科技局课题(18SXHZ0381)~~
关键词
食管鳞癌
酪氨酸蛋白激酶-7
人表皮生长因子受体2
微小染色体维持蛋白5
Esophageal squamous cell carcinoma
Protein tyrosine kinase 7
Human epidermal growth factor receptor 2
Mini chromosome maintenance
