摘要
目的探讨肾康丸对慢性间歇性缺氧致肾损伤大鼠氧化应激(OS)及炎症反应的影响。方法采用间歇性缺氧/再氧合动物舱建立慢性间歇性缺氧(CIH)大鼠模型,将CIH大鼠随机分为模型组(n=10)、肾康丸组(n=10)及常规条件生长的正常组(n=10)。采用ELISA试剂盒检测各组SD大鼠血清超氧化物歧化酶(SOD)及丙二醛(MDA)水平,运用肾脏称重法比较三组大鼠肾体比,HE染色病理切片观察肾组织变化,qPCR检测肾组织低氧诱导因子-1α(HIF-1α)、Gu/ZnSOD、MnSOD基因表达量,酶联免疫法检测大鼠血清白细胞介素-6(IL-6)与肿瘤坏死因子-α(TNF-α)水平。结果三组大鼠肾体比差异不明显(P>0.05);药物干预后,模型组大鼠血清SOD低于正常组、而MDA、IL-6与TNF-α水平明显高于正常组(均P<0.05),且模型组大鼠肾脏表现出肾小球系膜与基底膜增生;与模型组比较,肾康丸组大鼠血清SOD升高,而MDA、IL-6与TNF-α水平明显降低(均P<0.05),且HIF-1α基因表达量下降,Gu/ZnSOD、MnSOD基因表达量上升(均P<0.05)。结论肾康丸能降低CIH所致大鼠肾损伤的氧化应激与炎症反应。
ObjectiveTo explore the effects of Shenkang pill on oxidative stress and inflammatory response in rats with chronic intermittent hypoxia-induced renal injury. MethodsThe rat model of chronic intermittent hypoxia (CIH) was established by intermittent hypoxia/reoxygenation animal compartment. CIH rats were randomly and evenly divided into model group (10 rats) and Shenkang pill group (10 rats), and the rats with normal growth conditions were included in normal group (10 rats). Levels of serum superoxide dismutase (SOD) and malondialdehyde (MDA) in SD rats were detected by ELISA kit. The renal-body weight ratios of three groups were compared by renal weighting method. Renal tissue changes were observed by HE staining pathological section. qPCR was used to detect the genes expression levels of renal tissue hypoxia-inducible factor-1α(HIF-1α), Gu/ZnSOD and MnSOD. Levels of rats serum interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) were detected by enzyme-linked immunosorbent assay. ResultsThere was no significant difference in renal-body weight ratio among the groups ( P >0.05). The serum SOD level in model group was lower than that in normal group while the levels of MDA, IL-6 and TNF-α were significantly higher than those in normal group ( P <0.05), and the kidneys of model group showed mesangial membrane and basement membrane hyperplasia. Compared with model group, the levels of serum SOD, MDA, IL-6 and TNF-α in Shenkang pill group were significantly decreased ( P <0.05), and the expression level of HIF-1α gene was decreased while the expression levels of Gu/ZnSOD and MnSOD genes were increased ( P <0.05). ConclusionShenkang pill can reduce the oxidative stress and inflammatory response of rats with CIH-induced renal injury.
作者
许晓丽
何志婷
刘琼
赵静
冯玲
XU Xiaoli;HE Zhiting;LIU Qiong;ZHAO Jing;FENG Ling(Department of Nephrology, The Ninth Hospital of Xi'an, Xi'an 710054, China)
出处
《西部医学》
2019年第8期1185-1189,共5页
Medical Journal of West China
基金
陕西省自然科学基础研究计划项目(2014JM2-8160)
关键词
大鼠肾损伤
肾康丸
氧化应激
炎症反应
Rat renal injury
Shenkang pill
Oxidative stress
Inflammatory response
