摘要
为建立养心安神颗粒质量控制标准,采用薄层色谱法(TLC)对养心安神颗粒中的黄芪、人参、甘草、五味子、川芎、当归、酸枣仁7味药材进行了鉴别,对复方中君药黄芪中的有效成分黄芪甲苷采取高效液相色谱法(HPLC)进行了测定.结果表明黄芪、人参、五味子等7味药材的薄层色谱主斑点清晰,分离良好,阴性无干扰;黄芪甲苷在0.9884~5.9304μg之间线性良好,平均加样回收率为98.07%.建立的TLC和HPLC方法可靠、准确、重现性好,可全面控制养心安神颗粒的质量.
To establish the quality control standard of Yangxin Anshen granules,TLC was applied to the qualitative identification of 7 herbs including astragalus,ginseng and schisandra chinensis granules.HPLC method was used to determine the effective component of astragaloside.The results showed that the main spots were clear and the separation was good.Astragaloside was well linear at 0.988 4~5.930 4μg,with an average sample recovery of 98.07%.The established TLC and HPLC methods were reliable,accurate and reproducible,which could comprehensively control the quality of Yangxin Anshen granules.
作者
孙永慧
张静
周婕
SUN Yong-hui;ZHANG Jing;ZHOU Jie(Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2019年第3期274-279,共6页
Journal of Harbin University of Commerce：Natural Sciences Edition
关键词
养心安神颗粒
质量标准
薄层色谱
高相液相色谱
Yangxin Anshen granules
quality standard
high performance liquid chromatography
thin layer chromatography
