摘要
目的了解辽宁省的肠道病毒71型的流行特征并对VP1区基因特征进行分析。方法用2008—2018年辽宁省14个市送检的手足口病患者的阳性标本,通过细胞培养法分离肠道病毒,提取病毒RNA,用RT-PCR法对肠道病毒VP1区基因扩增和序列测定。利用BLAST对测序结果比对后确定病毒基因型别。对鉴定为EV71的分离株进行VP1区同源性分析,并构建系统进化树。结果辽宁省在2008—2018年间实验室总计收到阳性标本24749份,其中最多的是其它肠道病毒(43.77%);其次是CA16(32.67%);EV71(23.2%)。随机原则选取66株EV71毒株,核苷酸同源性为94.80%~99.80%,氨基酸的同源性为98.50%~100.00%;与C4a参考株的同源性最高,核苷酸的同源性为94.00%~98.00%,氨基酸的同源性为98.50%~100.00%;系统进化树分析表明,辽宁省EV71分离株为C4a基因亚型。结论EV71是引起辽宁省手足口病的重要病原体,是引起重症及死亡病例的优势病原;EV71分离株为C4a基因亚型,并且在辽宁省内有多个传播链共同流行。
Objective To understand the epidemic characteristics of enterovirus 71 in Liaoning Province and to analyze the genetic characteristics of VP1 region. Methods From 2008 to 2018, the positive samples of hand-foot-mouth disease patients from 14 cities in Liaoning Province were used to isolate enterovirus by cell culture, and viral RNA was extracted. RT-PCR was used to amplify the sequence of enterovirus VP1 region. Sequencing results were analyzed by BLAST. The isolates identified as EV71 were analyzed for homology in VP1 region and phylogenetic tree was constructed. Results From 2008 to 2018, a total of 24 749 positive samples were received, the majority of which were other enteroviruses, accounting for 43.77%, followed by CA16, accounting for 32.67%, while EV71 accounted for 23.2%. Sixty-six strains of EV71 were randomly selected and analyzed. The nucleotide homology was 94.8% to 99.8% and amino acid homology was 98.5%-100%. The homology with reference strain C4a was the highest, with the homology of nucleotide and amino acid being 94% to 98% and 98.5%-100%, respectively. Phylogenetic tree analysis indicated that EV71 isolates in Liaoning Province were C4a subtypes. Conclusion EV71 was an important pathogen causing hand-foot-mouth disease in Liaoning Province. EV71 isolates were subtypes of C4a, and there were multiple transmission chains in Liaoning Province.
作者
于伟
王伶
雷露
张倩
毛玲玲
姚文清
杨佐森
YU Wei;WANG Ling;LEI Lu;ZHANG Qian;MAO Lingling;YAO Wenqing;YANG Zuosen(Liaoning Center for Disease Control and Prevention,Shengyang 110005,China)
出处
《公共卫生与预防医学》
2019年第4期37-41,共5页
Journal of Public Health and Preventive Medicine
基金
辽宁省特色传染病病原谱流行规律研究(2017ZX10103007)
关键词
手足口病
肠道病毒71型
基因特征
Hand, foot and mouth disease
Enterovirus 71
Genetic characteristics
