摘要
目的:探讨艾司西酞普兰对强迫症大鼠胶质细胞源性神经营养因子(glial cell - derived neurotrophic factor,GDNF)、脑源性神经营养因子(brain - derived neurotrophic factor,BDNF)水平及脑组织五羟色胺(serotonin,5 - HT)含量的影响。方法:将42只大鼠根据随机数字法分为对照组、模型组(建立强迫症模型)和艾司西酞普兰组(建立强迫症模型+艾司西酞普兰治疗),每组14只。采用大鼠旷场法测试大鼠行为,采用酶联免疫吸附实验(ELISA)法测定血清GDNF和BDNF水平,采用Western blotting法测定脑组织GDNF和BDNF含量,采用高效液相结合电化学法测定脑组织5 - HT含量。结果:模型组和艾司西酞普兰组参观地点、旋转频率、不动持续时间均低于对照组( P< 0.05),运动总距离、移动频率均高于对照组( P< 0.05);艾司西酞普兰组参观地点、旋转频率、不动持续时间均高于模型组( P< 0.05),运动总距离、移动频率低于模型组( P< 0.05)。3组中心区频率和中心区持续时间比较差异均无统计学意义( P> 0.05)。血清、脑组织GDNF及BDNF水平模型组和艾司西酞普兰组均低于对照组( P< 0.05),艾司西酞普兰组均高于模型组( P< 0.05)。模型组和艾司西酞普兰组脑组织5 - HT水平均低于对照组( P< 0.05),艾司西酞普兰组脑组织5 - HT水平高于模型组( P< 0.05)。结论:艾司西酞普兰可升高强迫症大鼠血清GDNF、BDNF水平以及脑组织GDNF、 BDNF 、5 - HT含量,从而发挥对强迫症的治疗作用。
Objective: To investigate the effect of escitalopram on the levels of glial cell- derived neurotrophic factor(GDNF), brain- derived neurotrophic factor(BDNF) and serotonin(5- HT) content in brain tissue of obsessive- compulsive disorder rats. Methods: Forty- two rats were divided into control group, model group(established obsessive- compulsive disorder model) and escitalopram group(established obsessive- compulsive disorder model + escitalopram treatment) according to the random table method, with 14 rats in each group. The rats′ behaviors were tested by rat open field test. The serum GDNF and BDNF levels were measured by enzyme linked immunosorbent assay (ELISA). The levels of brain GDNF and BDNF protein were determined by Western blotting. The level of 5- HT in brain tissue was determined by HPLC using electrochemical method. Results: The site of visit, rotation frequency and immobility duration of the model group and the escitalopram group were lower than those of the control group ( P< 0.05), and the total distance and frequency of movement were higher than those of the control group ( P< 0.05).The site of visit, rotation frequency and immobility duration of the escitalopram group were higher than those of the model group ( P< 0.05), and the total distance and frequency of movement in the escitalopram group were lower than that in the model group ( P< 0.05).There were no significant differences in the frequency of the central area and the duration of the central area among the three groups ( P> 0.05).The levels of serum and brain tissue GDNF and BDNF in the model group and the escitalopram group were lower than those in the control group ( P< 0.05).The serum and brain tissue GDNF and BDNF levels in the escitalopram group were higher than that in the model group ( P< 0.05).The 5- HT levels in brain tissue of the model group and the escitalopram group were lower than those in the control group ( P< 0.05).The level of 5- HT in brain of the escitalopram group was higher than that of the model group ( P< 0.05). Conclusion: Escitalopram can increase serum and brain tissue GDNF and BDNF levels in obsessive- compulsive disorder rats, and increase the content of 5- HT in brain tissue to exert its therapeutic effect on obsessive- compulsive disorder.
作者
郭慧荣
王亚丽
张延妍
马全钢
王源莉
吕培培
李淑英
GUO Huirong;WANG Yali;ZHANG Yanyan;MA Quangang;WANG Yuanli;L Peipei;LI Shuying(Department of Psychiatry,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《东南大学学报（医学版）》
CAS
2019年第4期677-681,共5页
Journal of Southeast University(Medical Science Edition)
基金
河南省科技厅资助项目(172102310578)
