摘要
以黄花风铃木(Tabebuiachrysantha)幼苗为试验材料,采用L16(4^5)正交试验设计,测定并比较了各个浓度组合处理下黄花风铃木幼苗的生长指标以及生理特性,研究3种植物生长调节剂(GGR、IAA、PP333)不同组合对黄花风铃木幼苗的生长和抗性生理交互作用影响,得出提高黄花风铃木幼苗的抗性的最佳浓度组合。结果表明,11号处理(GGR浓度为400 mg·L^-1、IAA浓度为400 mg·L^-1、PP333浓度为400 mg·L^-1)根系各生长指标大于其他组合处理,地径增长量8号处理最大,11号处理次之。8号、11号、15号苗高生长量高于其他处理。3种植物生长调节剂中GGR对黄花风铃木幼苗根系生长,地径增长和苗高增长起主导作用,IAA和PP333作用不明显。11号处理幼苗的可溶性蛋白、超氧化物歧化酶(SOD)活性均大于其他处理组,丙二醛(MDA)含量最低,而过氧化物酶(POD)活性则是4、11和12号处理明显高于其他处理组合。经分析得出GGR对黄花风铃木幼苗的可溶性蛋白、SOD以及POD起主导作用,IAA和PP333作用不明显。PP333对黄花风铃木幼苗MDA含量影响显著,IAA和GGR对MDA含量作用不明显。通过对黄花风铃木幼苗各个指标综合分析结果表明,不同生长调节剂的适宜浓度组合为11号处理(GGR浓度为400 mg·L^-1、IAA浓度为400 mg·L^-1、PP333浓度为400 mg·L^-1),在此浓度下黄花风铃木幼苗生长情况及抗性生理最佳。本实验揭示GGR、IAA和PP333 3种植物生长调节剂交互作用对黄花风铃木幼苗的影响,为植物生长调节剂在提高园林植物抗逆性,培育抗性品种等方面的运用提供更多的理论支持和依据。
In order to study the interaction of combinations of three plant growth regulators (GGR, IAA and PP333) on the growth and resistance physiological of Tabebuia chrysantha seedlings, and obtain the best concentration combination for improving the resistance of Tabebuia chrysantha seedlings, we chosen Tabebuia chrysantha seedlings as experimental materials, used L16(4^5) orthogonal experiment design, measured and compared the growth and physiological characteristics of Tabebuia chrysantha seedlings. The results showed that root growth index and growth amount of seedling height with No. 11 treatment (GGR 400 mg·L^-1, IAA 400 mg·L^-1, PP333 400 mg·L^-1) was greater than those with other combination treatments, and which had the largest growth ground diameter with No.8 treatment, followed by No. 11. Among the three plant growth regulators, GGR played a leading role in the root growth index, growth amount of seedling height and diameter of Tabebuia chrysantha seedlings, but IAA and PP333 had no significant effect. The soluble protein and superoxide dismutase (SOD) activity in the seedlings with No. 11 treatment were greater than those with other treatment groups, while the content of malondialdehyde (MDA) in the seedlings with No. 11 treatment was the lowest, and the activities of peroxidase (POD) in the seedlings with No. 4, No. 11 and No. 12 treatments were significantly higher than those with other treatments. The results showed that: GGR plays a leading role in the soluble protein, SOD activity and POD activity of the Tabebuia chrysantha seedlings,and IAA and PP333 have no obvious effect;PP333 has significant effect on the content of MDA in Tabebuia chrysantha seedlings, but IAA and GGR have no significant effect on the content of MDA. The results of the comprehensive analysis of each index of Tabebuia chrysantha seedlings showed that the suitable concentration combination of different growth regulators is No. 11 treatment (GGR 400 mg L^-1, IAA 400 mg L^-1, PP333 400 mg L^-1). Under this concentration, the root growth and resistance physiology of Tabebuia chrysantha seedlings are the best. In this study,we preliminarily studied the interaction of GGR, IAA and PP333 on the Tabebuia chrysantha seedling, which will provide more theoretical support and basis for the application of plant growth regulators in improving stress tolerance of garden plants and breeding resistant varieties.
作者
黄杰
施福军
李曼清
王凌晖
滕维超
HUANG Jie;SHI Fujun;LI Manqing;WANG Linghui;TENG Weichao(College of Forestry, Guangxi University, Nanning 530004;Nanning Arboretum, Nanning 530000;Central and South China Fast Growing Timber Breeding Key Laboratory of State Forestry Administration, Nanning 530000)
出处
《安徽农业大学学报》
CAS
CSCD
2019年第3期440-447,共8页
Journal of Anhui Agricultural University
基金
国家自然科学基金项目(31360174)
广西林业科技项目(桂林科字[2012]第25号)共同资助
关键词
黄花风铃木
植物生长调节剂
生长特性
抗性生理
最适浓度组合
Tabebuia chrysantha
plant growth regulator
growth characteristics
resistance physiology
optimum concentration combination
