摘要
目的:观察助阳通便膏对功能性便秘大鼠肠道酶活性的影响,进一步阐明功能性便秘的发生机制,以便为其有效防治提供新靶点。方法:将100只昆明大鼠,雌雄各半,随机分成5组(正常组、模型组、助阳通便膏组、助阳通便汤组、麻仁软胶囊组),每组20只,除正常组外均予复方地芬诺酯进行大鼠便秘模型造模。造模成功后,各组均予2周治疗后,对大鼠肠道碳末推进率进行检测,并应用免疫组化的方法探讨助阳通便膏方对便秘模型大鼠肠道酶活性的影响。结果:模型组大鼠道碳末推进率较正常组明显减低(P<0.05),24 h排便量明显降低(P<0.01)。助阳通便膏组肠道碳末推进率较模型组显著延长(P<0.01),24 h排便量接近正常组,明显高于模型组(P<0.01)。助阳通便汤组肠道碳末推进率及24 h排便量介于助阳通便膏组与麻仁软胶囊组之间。麻仁软胶囊组肠道碳末推进率较模型组延长(P<0.05),24 h排便量小于助阳通便汤组(P>0.05),高于模型组(P<0.05)。与模型组相比,助阳通便膏组、助阳通便汤组、麻仁软胶囊组均能升高大鼠蛋白酶和纤维素酶活性,降低木聚糖酶活性,且助阳通便膏组更明显。结论:助阳通便膏可通过调节肠道酶活性,增加粪便含水量,改善肠道功能,治疗效果优于汤剂,并且在治疗便秘的过程中,与麻仁软胶囊比较,有更好治疗作用。
Objective:To observe the effect of Zhuyang Tongbian Ointment on intestinal enzyme activity in rats with functional constipation, and further elucidate the mechanism of functional constipation in order to provide new targets for its effective prevention and treatment.Methods:A hundred Kunming rats, half male and half female, were randomly divided into 5 groups(normal group, model group, Zhuyang Tongbian Ointment group, Zhuyang Tongbian Decoction group, Maren Capsules group), 20 rats in each group. Models were set up with compound diphenoxylate in addition to the normal group. After successful modeling, each group was treated for 2 weeks, and the intestinal propellant rate of carbon powder of the rats was tested. The immunohistochemical was used to study the effect of Zhuyang Tongbian Ointment on intestinal enzyme activity in rats with constipation model.Results:In the model group, the propellant rate of carbon powder was significantly lower in the rats than that in the normal group(P<0.05), and the 24-hour defecation was significantly lower(P<0.01). The intestinal propellant rate of carbon powder of the Zhuyang Tongbian Ointment group was significantly higher than that of the model group(P<0.01). The 24-hour bowel movement was close to that of the normal group, which was significantly higher than that of the model group(P<0.01). The intestinal propellant rate of carbon powder and the 24-hour defecation volume of the Zhuyang Tongbian Decoction group were between the Zhuyang Tongbian Ointment group and the Maren Capsules group. The propellant rate of carbon powder of the Maren Capsules group was higher than that of the model group(P<0.05), and the 24-hour defecation volume was smaller than that of the Zhuyang Tongbian Decoction group(P>0.05), which was higher than that of the model group(P<0.05). Compared with the model group, the Zhuyang Tongbian Ointment group, the Zhuyang Tongbian Decoction group and the Maren Capsules group can increase the activity of protease and cellulase in rats and reduce the activity of xylanase,and the Zhuyang Tongbian Ointment group is more obvious.Conclusion:Zhuyang Tongbian Ointment can improve intestinal function and increase fecal water content by regulating intestinal enzyme activity. The therapeutic effect is better than the decoction and in the process of treating constipation, compared with Ma ren Capsules, there is a better therapeutic effect.
作者
石宇
于修永
张虹玺
SHI Yu;YU Xiuyong;ZHANG Hongxi(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110003,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第8期1939-1942,共4页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省自然科学基金项目(20180550953)
