摘要
旨在探讨湿筛分过程中用水量、上样量及筛分时间对猪颗粒饲料、肠道食糜及粪便平均粒径测定的影响,为猪颗粒饲料、肠道食糜及粪便粒径的测定提供参考。本研究选用标准筛孔径分别为2.0、1.0、0.5、0.25、0.106和0.072 mm套筛进行湿筛分测定平均粒径。采用3×2×2三因素完全随机设计,筛分过程中用水量设1、1.25或1.5 L;上样量分别为颗粒料7.5或10 g,回肠食糜15或30 g,粪便10或20 g;筛分时间分别为4或5 min。每种类型样品共12个处理。结果表明:1)在各层筛上物质量占比中,随用水量的增加,颗粒料、回肠食糜及粪便中1 mm以上颗粒质量占比显著下降(P<0.05),可溶物质量占比显著上升(P<0.05);随上样量的增加,1 mm以上颗粒质量占比显著上升(P<0.05),可溶物或0.072 mm以下颗粒质量占比显著下降(P<0.05);而筛分时间对各层筛筛上物质量的占比均没有显著影响。2)用水量、上样量对颗粒料、回肠食糜及粪便平均粒径的测定均有极显著影响(P<0.01),筛分时间仅对食糜测定结果有显著影响(P<0.05),但仅相差4μm。用水量为1 L时测定的平均粒径均显著高于1.25和1.5 L(P<0.05),而1.25和1.5 L仅在粪便的测定结果有显著差异(P<0.05),在颗粒料和回肠食糜的测定结果无显著差异。颗粒饲料上样量7.5~10 g,食糜上样量15~30 g,粪便上样量10~20 g时,粒径测定结果的差异均在12μm以内。综上,在测定猪颗粒饲料、食糜及粪便的粒径时,颗粒饲料上样量7.5~10 g,食糜上样量15~30 g,粪便上样量10~20 g,用水量1.25 L,筛分时间4 min获得的测定结果相对稳定。
This study was conducted to investigate the effects of water volume,sample weight and sieving time on the mean particle size of pelleted diet,digesta and feces during wet-sieving,and to provide a reference for the determination of particle size for pelleted diet,digesta and feces of pig.The mean particle size was assayed with wet-sieving using a set of 6 sieves sized 2.0,1.0,0.5,0.25,0.106 and 0.072 mm.The water volume of 1,1.25 or 1.5 L,sample weight of 7.5 or 10 g for pelleted diet,15 or 30 g for ileal digesta,10 or 20 g for feces and sieving time of 4 or 5 min were used in a 3×2×2 factorial completely randomized arrangement.There were 12 treatments for each type of sample.The effect of wet-sieving conditions on the measurement of geometric mean particle size of samples was investigated.The results showed that:1)Increment of water volume significantly decreased the proportion of the mass content of the particles more than 1 mm in size(P<0.05),but significantly increased the proportion of the mass content of soluble matter in pelleted diet,ileal digesta and feces(P<0.05);Increment of the sample weight significantly increased the proportion of the mass content of the particles more than 1 mm in size(P<0.05),but significantly decreased the proportion of the mass content of soluble matter or particles less than 0.072 mm(P<0.05).However,there was no significant effect of sieving time on the proportion of the mass content on each sieves.2)Water volume and sample weight extremely significantly affected the determination of mean particle size for pelleted diet,ileal digesta and feces(P<0.01),but significant effect of the sieving time was only observed on the determination of ileal digesta(P<0.05).The determined value of mean particle size was significantly higher in 1 L of water volume than 1.25 or 1.5 L(P<0.05).Moreover,particle size was significantly different between 1.25 and 1.5 L of water volume for feces(P<0.05),but there was no significant differences between 1.25 and 1.5 L of water volume for ileal digesta and pelleted diet.The sample weight of pelleted diet ranged from 7.5 to 10 g,ileal digesta from 15 to 30 g,and feces from 10 to 20 g led to the difference of less than 12μm in determined particle size.In summary,in the determination of particle size for pelleted diet,digesta and feces of pig using wet-sieving method,the sample weight of pelleted diet ranged from 7.5 to 10 g,ileal digesta from 15 to 30 g,feces from 10 to 20 g,1.25 L of water volume and 4 min of sieving time are the optimized condition to obtain a relatively stable result.
作者
高庆涛
赵峰
张虎
王亚
GAO Qingtao;ZHAO Feng;ZHANG Hu;WANG Ya(State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第8期1614-1624,共11页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
中国农业科学院科技创新工程(ASTIP-IAS07)
广东温氏食品集团股份有限公司合作项目(2018-YF-01)
关键词
湿筛分
颗粒料
食糜
粪便
粒径
筛分条件
wet-sieving
pelleted diet
digesta
feces
particle size
sieving conditions
