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2015年-2017年黄山市流感病毒核酸检测结果分析 被引量:6

Analysis of influenza virus nucleic acid test results in Huangshan from 2015 to 2017
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摘要 目的分析黄山市2015年-2017年流感样病例(influenza like illness,ILI)流感病毒特异性核酸监测结果,为本地区制定流感防控措施提供实验室依据。方法采用荧光定量PCR对ILI咽拭子标本进行流感病毒特异性核酸检测。结果 2015年-2017年ILI流感病毒核酸总阳性率为17. 95%,病毒型别为新甲H1型、季H3型、B-Victoria(BV)、B-Yamagata(BY),以季H3型为主(61. 1%);2月-3月、7月-8月和12月阳性检出率较高,新甲H1型和BV主要在1月-5月检出,BY在1月-3月和10月-12月检出,季H3型全年均可检出,7月-8月和11月-12月是检出高峰;各年龄组ILI流感核酸阳性率差异有统计学意义(P <0. 05),5岁~14岁组阳性率最高(22. 03%),0岁~4岁组最低(9. 05%);流感阳性患者以学生和干部职员所占比例大;不同性别ILI阳性率差异无统计学意义(P> 0. 05)。结论 2015年-2017年黄山市流感流行季节主要在冬春季和夏季,以季H3型为优势毒株;4种流感亚型流行时间不同;学生和干部职员是今后流感防控重点人群。 Objective To analyze the results of influenza virus specific nucleic acid monitoring of influenza-like cases in Huangshan City from 2015 to 2017,so as to provide laboratory basis for the formulation of influenza prevention and control measures in the region. Methods Fluorescent quantitative PCR was used to detect influenza virus-specific nucleic acids in throat swabs of ILI cases. Results The total positive rate of ILI influenza virus nucleic acid was 17. 95% from 2015 to 2017,and the virus types were new H1,seasonal H3,B-Victoria( BV),and Yamagata( BY),with season H3 as the dominant( 61. 1%);The positive detection rate was higher from February to march,July to August and December. New H1 and BV were mainly detected from January to May. BY was detected from January to March and from October to December. Seasonal H3 can be detected throughout the year,with July-August and Novenber-December as the detection peak;The difference in positive rate of influenza nucleic acid detection in all age groups was statistically significant( P < 0. 05). The highest positive rate was in the age group of 5-14( 22. 03%) and the lowest was in the age of 0-4( 9. 05%);Influenza positive patients account for a large proportion of students and cadres;There was no statistical significance on the difference in ILI positive rate between different genders( P > 0. 05). Conclusion The influenza epidemic season in Huangshan City from 2015 to 2017 is mainly in winter,spring and summer,with H3 influenza virus as the dominant strain;The epidemic time of four influenza subtypes is different;Students and cadres are the key groups for influenza control in the future.
作者 黄玲玲 胡婉琦 程婧 方汀 HUANG Ling-ling;HU Wan-qi;CHENG Jing;FANG Ting(Huangshan Center for Disease Control and Prevention, Huangshan, Anhui 24500, China)
出处 《中国卫生检验杂志》 CAS 2019年第14期1712-1714,1723,共4页 Chinese Journal of Health Laboratory Technology
关键词 流感病毒 核酸检测 荧光定量PCR 防控措施 Influenza virus Nucleic acid detection Fluorescent quantitative PCR Prevention and control measures
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