摘要
为研究外膜蛋白YbjX对大肠杆菌致病作用的影响,本研究利用λ-Red同源重组系统构建了狐肺炎大肠杆菌(HBCLE-12)ybjX基因缺失突变株(HBCLEΔybjX),并对其生物学特性进行分析,探索YbjX蛋白在大肠杆菌致病过程中的作用。结果表明,缺失株HBCLEΔybjX与野生型菌株和回补菌株相比,其生长速度、生化特性无明显差异,但其运动能力和血清内存活能力显著下降,对多种抗生素的敏感性明显增强。动物试验表明,ybjX基因缺失突变株对小鼠的致病力显著下降。本研究为进一步阐释大肠杆菌致病机制和疫苗设计奠定基础。
To investigate the roles of outer membrane protein(Omp)YbjX in pathogenic processes of E.coli.λ-Red homologous recombination was used for knockout of ybjX gene,and comparison of growth characteristics,biochemical characteristics and bacterial virulence were performed for analysis the change between the HBCLE-12wild type strain and ybjX knockout mutant.The data demonstrated that,the YbjX-deficient mutant showed no differences in growth characteristics and biochemical characteristics with wild type strains and complemented strains.But compared with the wild type and complemented strains,the mutant displayed sharply decreased survival rate in both foxes and mouse serum,attenuated migration in 0.25%agar,and significantly increased sensitivity to multiple antibotics.The apparently attenuated virulence was also observed in YbjX-inactivation mutant.The experiment generated a YbjX-deficient mutant of E.coli,providing foundation for exploring underlying pathogenic mechanism of E.coli and vaccine design.
作者
张志强
苏硕青
李巧玲
肖丽荣
李永慧
黄翠琴
段滇宁
吴同垒
高云航
ZHANG Zhi-qiang;SU Shuo-qing;LI Qiao-ling;XIAO Li-rong;LI Yong-hui;HUANG Cuiqin;DUAN Dian-ning;WU Tong-lei;GAO Yun-hang(Hebei Normal University of Science &Technology,Qinhuangdao,Hebei 066004,China;The Second Hospital of Qinhuangdao, Changli,Hebei 066600,China;Fujian Key Laboratory of Prevention and Control of Livestock Contagious Disease & Biotechnology,Longyan,Fujian 364000,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2019年第7期1291-1296,共6页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31802186)
福建省家畜传染病防治与生物技术重点实验室开放基金课题资助项目(2016KL02)
河北科技师范学院博士启动基金资助项目(2015YB002)
河北省高等学校科学技术研究资助项目(ZD2016067)
秦皇岛市科技创新平台建设运行经费资助项目(201702B001)
中国博士后科学基金资助项目(2017M611935)
