摘要
基于植物DNA条形码技术建立淀粉及其加工制品成分鉴别检测方法。通过提取淀粉5大物种苜蓿、马铃薯、木薯、番薯和玉米的基因组DNA为模板,分别以ITS2、matK、rbcL和trnH-psbA基因通用引物进行聚合酶链式反应扩增,并将测序结果提交GenBank数据库BLAST比对,评价不同植物DNA条形码序列对其鉴别能力。筛选出ITS2+trnH-psbA为较适组合序列,并对自行采购的5个淀粉样品和4个粉条样品进行检测。
This study reported on the application of plant DNA barcoding technique to detect adulteration of starch and starch products. Genomic DNA samples were extracted from alfalfa, potato, cassava, sweet potato and maize and were used as templates for PCR amplification with the universal primers ITS2, matK, rbcL and trnH-psbA. The sequences were aligned using the basic local alignment search tool(BLAST) with those deposited in the GenBank database. The identification abilities of 4 DNA fragments were compared by success rate. Five starch samples and 4 vermicelli samples we collected were detected using the group of ITS2 + trnH-psbA with higher efficiency.
作者
陈佳
王爽
周巍
张岩
桑亚新
CHEN Jia;WANG Shuang;ZHOU Wei;ZHANG Yan;SANG Yaxin(College of Food Science and Technology, Agricultural University of Hebei, Baoding 071000, China;Shandong Institute of Standardization, Jinan 250000, China;Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第16期281-285,共5页
Food Science
基金
“十三五”国家重点研发计划重点专项（2017YFC1601700）
关键词
DNA条形码
淀粉
粉条
掺假鉴定
DNA barcoding
starch
vermicelli
adulteration detection
