摘要
随着生命科学研究的深入发展,对大通量、大面积、大视野样品的分析越来越需要。玻片扫描是利用全自动显微镜扫描系统,结合虚拟切片软件,把传统切片进行扫描、无缝拼接,生成一整张全视野的数字切片。为促进虚拟切片扫描类大型仪器给教学和科研工作提供更好的共享服务,通过介绍OLYMPUS VS120虚拟数字切片扫描系统的硬件配置、成像特点和软件功能,结合具体使用实例探讨该仪器在组织病理学、神经生物学、发育生物学等研究领域尤其大视野拼图的重要应用。总结仪器使用过程中遇到的问 题,分析造成的原因,提出解决方案,为数字切片扫描系统的共享使用提供了借鉴和参考。
With the further development of life science research, the analysis of large-flux, large-area and large-field samples is increasingly carried out. The slide scanning system uses a fully automatic microscope scanning system, combines with a virtual slicing software, to scan and seamlessly splice traditional slices to generate a full-field digital slice. In order to promote virtual slice scanning instruments to provide better sharing services for teaching and scientific research, the hardware configuration, imaging characteristics and software functions of OLYMPUS VS120 virtual digital slice scanning system are introduced, and its application in the large-image stitching in the fields of histopathology, neurobiology and developmental biology is discussed with practical examples. This paper summarizes the problems encountered during the use of the instrument, analyzes the causes, proposes solutions, and provides reference for the shared use of the digital slice scanning system.
作者
尹伟
刘双双
方三华
YIN Wei;LIU Shuangshuang;FANG Sanhua(School of Medicine, Zhejiang University, Hangzhou 310058, China)
出处
《实验室研究与探索》
CAS
北大核心
2019年第8期105-109,共5页
Research and Exploration In Laboratory
基金
2019年度浙江省分析测试项目(LGC19H090001)
关键词
虚拟数字切片
扫描系统
大视野拼图
virtual digital slice
scanning system
large image stitching
