摘要
目的:建立金佛止痛丸中芍药苷、延胡索乙素、三七皂苷R 1的含量测定方法。方法:采用HPLC法,所用色谱柱为沃特世XBRIDGE-C 18 (250 mm×4.6 mm,5 μm),流动相A为乙腈-甲醇(1∶1),流动相B为0.01 mol/L磷酸二氢钠,梯度洗脱;流速为 1.0 ml/min,分段变波长测定,进样20 μl。结果:芍药苷、延胡索乙素、三七皂苷R 1的线性范围分别为 10~ 100 μg/ml( r= 0.999 7)、0.2~2 μg/ml( r= 0.999 0)和2.5~25 μg/ml( r= 0.999 3),回收率分别为99.83%(RSD 为 0.24%, n= 9)、101.18%(RSD 为 1.24%, n= 9)和99.96%(RSD 为 0.79%, n= 9)。结论:该测定方法高效、准确,可用于金佛止痛丸的质量控制。
Objective: To establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of paeoniflorin, tetrahydropalmatine, notoginsenoside R 1 in Jinfo Zhitong Pills. Methods: The analysis was performed on waters XBRIDGE-C 18 (250 mm×4.6 mm, 5 μm) column with mobile phase consisted of acetoneitrile-methanol-0.01 mol/L sodium dihydrogen phosphate solution. The flow rate was 1.0 mL/min for gradient elution. Results: The responses of paeoniflorin, tetrahydropalmatine, notoginsenoside R 1 were liner in the ranges of 10~100 μg/ml ( r= 0.999 7), 0.2~2 μg/ml ( r= 0.999 0), 2.5~ 25 μg/ml ( r= 0.999 3), respectively. The average recoveries for paeoniflorin, tetrahydropalmatine, notoginsenoside R 1 were 99.83%(RSD was 0.24%, n =9), 101.18%(RSD was 1.24%, n= 9), 99.96%(RSD was 0.79%, n= 9), respectively. Conclusion: The established method is accurate and reproducible, and can be used as quality control for Jinfo Zhitong Pills.
作者
王银
邱连建
Wang Yin;Qiu Lianjian(Shenzhen Longgang District Hospital of Traditional Chinese Medicine,Guangdong 518033)
出处
《天津药学》
2019年第4期29-31,共3页
Tianjin Pharmacy
